Fabrication of cell-containing hydrogel microstructures inside microfluidic devices that can be used as cell-based biosensors

Won Gun Koh, Michael V. Pishko

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

This paper describes microfluidic systems containing immobilized hydrogel-encapsulated mammalian cells that can be used as cell-based biosensors. Mammalian cells were encapsulated in three-dimensional poly(ethylene glycol)(PEG) hydrogel microstructures which were photolithographically polymerized in microfluidic devices and grown under static culture conditions. The encapsulated cells remained viable for a week and were able to carry out enzymatic reactions inside the microfluidic devices. Cytotoxicity assays proved that small molecular weight toxins such as sodium azide could easily diffuse into the hydrogel microstructures and kill the encapsulated cells, which resulted in decreased viability. Furthermore, heterogeneous hydrogel microstructures encapsulating two different phenotypes in discrete spatial locations were also successfully fabricated inside microchannels.

Original languageEnglish
Pages (from-to)1389-1397
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume385
Issue number8
DOIs
Publication statusPublished - 2006 Aug 1

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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