Abstract
This paper describes microfluidic systems containing immobilized hydrogel-encapsulated mammalian cells that can be used as cell-based biosensors. Mammalian cells were encapsulated in three-dimensional poly(ethylene glycol)(PEG) hydrogel microstructures which were photolithographically polymerized in microfluidic devices and grown under static culture conditions. The encapsulated cells remained viable for a week and were able to carry out enzymatic reactions inside the microfluidic devices. Cytotoxicity assays proved that small molecular weight toxins such as sodium azide could easily diffuse into the hydrogel microstructures and kill the encapsulated cells, which resulted in decreased viability. Furthermore, heterogeneous hydrogel microstructures encapsulating two different phenotypes in discrete spatial locations were also successfully fabricated inside microchannels.
| Original language | English |
|---|---|
| Pages (from-to) | 1389-1397 |
| Number of pages | 9 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 385 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2006 Aug 1 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
Cite this
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Fabrication of cell-containing hydrogel microstructures inside microfluidic devices that can be used as cell-based biosensors. / Koh, Won Gun; Pishko, Michael V.
In: Analytical and Bioanalytical Chemistry, Vol. 385, No. 8, 01.08.2006, p. 1389-1397.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fabrication of cell-containing hydrogel microstructures inside microfluidic devices that can be used as cell-based biosensors
AU - Koh, Won Gun
AU - Pishko, Michael V.
PY - 2006/8/1
Y1 - 2006/8/1
N2 - This paper describes microfluidic systems containing immobilized hydrogel-encapsulated mammalian cells that can be used as cell-based biosensors. Mammalian cells were encapsulated in three-dimensional poly(ethylene glycol)(PEG) hydrogel microstructures which were photolithographically polymerized in microfluidic devices and grown under static culture conditions. The encapsulated cells remained viable for a week and were able to carry out enzymatic reactions inside the microfluidic devices. Cytotoxicity assays proved that small molecular weight toxins such as sodium azide could easily diffuse into the hydrogel microstructures and kill the encapsulated cells, which resulted in decreased viability. Furthermore, heterogeneous hydrogel microstructures encapsulating two different phenotypes in discrete spatial locations were also successfully fabricated inside microchannels.
AB - This paper describes microfluidic systems containing immobilized hydrogel-encapsulated mammalian cells that can be used as cell-based biosensors. Mammalian cells were encapsulated in three-dimensional poly(ethylene glycol)(PEG) hydrogel microstructures which were photolithographically polymerized in microfluidic devices and grown under static culture conditions. The encapsulated cells remained viable for a week and were able to carry out enzymatic reactions inside the microfluidic devices. Cytotoxicity assays proved that small molecular weight toxins such as sodium azide could easily diffuse into the hydrogel microstructures and kill the encapsulated cells, which resulted in decreased viability. Furthermore, heterogeneous hydrogel microstructures encapsulating two different phenotypes in discrete spatial locations were also successfully fabricated inside microchannels.
UR - http://www.scopus.com/inward/record.url?scp=33746887011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746887011&partnerID=8YFLogxK
U2 - 10.1007/s00216-006-0571-6
DO - 10.1007/s00216-006-0571-6
M3 - Article
C2 - 16847626
AN - SCOPUS:33746887011
VL - 385
SP - 1389
EP - 1397
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 8
ER -