Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
Bibliographical noteFunding Information:
This work was financially supported by a Korea Science and Engineering Foundation (KOSEF) grant funded by the Korean Government (MOST) (Nos. R11-2007-050-00000-0 and R01-2007-000-10353-0 ), the Nano R&D program through the Korea Science and Engineering Foundation funded by the Ministry of Education, Science and Technology ( 2011-0019176 ), the Ministry of Knowledge Economy ( 10030051 ), the Korea Science and Engineering Foundation ( 2010-0029220 , 2009K001644 ), Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2011-0023064 ), and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea ( A110416 ).
All Science Journal Classification (ASJC) codes
- Pharmaceutical Science