Abstract
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
| Original language | English |
|---|---|
| Pages (from-to) | 417-425 |
| Number of pages | 9 |
| Journal | International Journal of Pharmaceutics |
| Volume | 427 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 May 10 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Pharmaceutical Science
Cite this
}
Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery. / Park, Hongkwan; Kim, Pyung Hwan; Hwang, Taewon; Kwon, Oh Joon; Park, Tae Joon; Choi, Sung Wook; Yun, Chae Ok; Kim, Jung-Hyun.
In: International Journal of Pharmaceutics, Vol. 427, No. 2, 10.05.2012, p. 417-425.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fabrication of cross-linked alginate beads using electrospraying for adenovirus delivery
AU - Park, Hongkwan
AU - Kim, Pyung Hwan
AU - Hwang, Taewon
AU - Kwon, Oh Joon
AU - Park, Tae Joon
AU - Choi, Sung Wook
AU - Yun, Chae Ok
AU - Kim, Jung-Hyun
PY - 2012/5/10
Y1 - 2012/5/10
N2 - Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
AB - Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
UR - http://www.scopus.com/inward/record.url?scp=84862779320&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862779320&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2012.01.050
DO - 10.1016/j.ijpharm.2012.01.050
M3 - Article
C2 - 22326298
AN - SCOPUS:84862779320
VL - 427
SP - 417
EP - 425
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
SN - 0378-5173
IS - 2
ER -