Fabrication of high quality cDNA microarray using a small amount of cDNA.

Chan Hee Park, Ha Jin Jeong, Jae Jun Jung, Gui Yeon Lee, Sang Chul Kim, Tae Soo Kim, Sang Hwa Yang, Hyun Cheol Chung, Sun Young Rha

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

DNA microarray technology has become an essential part of biological research. It enables the genome-scale analysis of gene expression in various types of model systems. Manufacturing high quality cDNA microarrays of microdeposition type depends on some key factors including a printing device, spotting pins, glass slides, spotting solution, and humidity during spotting. UsingEthe Microgrid II TAS model printing device, this study defined the optimal conditions for producing high density, high quality cDNA microarrays with the least amount of cDNA product. It was observed that aminosilane-modified slides were superior to other types of surface modified-slides. A humidity of 30+/-3% in a closed environment and the overnight drying of the spotted slides gave the best conditions for arraying. In addition, the cDNA dissolved in 30% DMSO gave the optimal conditions for spotting compared to the 1X ArrayIt, 3X SSC and 50% DMSO. Lastly, cDNA in the concentration range of 100-300 ng/ micro l was determined to be best for arraying and post-processing. Currently, the printing system in this study yields reproducible 9000 spots with a spot size 150 mm diameter, and a 200 nm spot spacing.

Original languageEnglish
Pages (from-to)675-679
Number of pages5
JournalInternational journal of molecular medicine
Volume13
Issue number5
Publication statusPublished - 2004 May

All Science Journal Classification (ASJC) codes

  • Genetics

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    Park, C. H., Jeong, H. J., Jung, J. J., Lee, G. Y., Kim, S. C., Kim, T. S., Yang, S. H., Chung, H. C., & Rha, S. Y. (2004). Fabrication of high quality cDNA microarray using a small amount of cDNA. International journal of molecular medicine, 13(5), 675-679.