Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Yeol Lee, Hyun Jong Lee, Kyung Jin Son, Won Gun Koh

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 m and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzyme-entrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.

Original languageEnglish
Pages (from-to)4476-4483
Number of pages8
JournalJournal of Materials Chemistry
Volume21
Issue number12
DOIs
Publication statusPublished - 2011 Mar 28

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability'. Together they form a unique fingerprint.

  • Cite this