Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

Yeol Lee, Hyun Jong Lee, Kyung Jin Son, Won-Gun Koh

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 m and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzyme-entrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.

Original languageEnglish
Pages (from-to)4476-4483
Number of pages8
JournalJournal of Materials Chemistry
Volume21
Issue number12
DOIs
Publication statusPublished - 2011 Mar 28

Fingerprint

Immunosensors
Hydrogel
Microarrays
Nanofibers
Hydrogels
Polyethylene glycols
Enzymes
Fabrication
Immunoglobulin G
Electrospinning
Substrates
Proteins
Immobilized Enzymes
Microstructure
Fibers
Polystyrenes
Biosensors
Lithography
Reaction rates
Assays

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Chemistry

Cite this

@article{abcb95fdee6b4e35b70429d17c3ee558,
title = "Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability",
abstract = "Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 m and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzyme-entrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.",
author = "Yeol Lee and Lee, {Hyun Jong} and Son, {Kyung Jin} and Won-Gun Koh",
year = "2011",
month = "3",
day = "28",
doi = "10.1039/c0jm03881d",
language = "English",
volume = "21",
pages = "4476--4483",
journal = "Journal of Materials Chemistry",
issn = "0959-9428",
publisher = "Royal Society of Chemistry",
number = "12",

}

Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability. / Lee, Yeol; Lee, Hyun Jong; Son, Kyung Jin; Koh, Won-Gun.

In: Journal of Materials Chemistry, Vol. 21, No. 12, 28.03.2011, p. 4476-4483.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fabrication of hydrogel-micropatterned nanofibers for highly sensitive microarray-based immunosensors having additional enzyme-based sensing capability

AU - Lee, Yeol

AU - Lee, Hyun Jong

AU - Son, Kyung Jin

AU - Koh, Won-Gun

PY - 2011/3/28

Y1 - 2011/3/28

N2 - Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 m and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzyme-entrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.

AB - Nanofiber-based protein microarrays were fabricated through a combination of electrospinning and hydrogel lithography. Electrospinning generated polystyrene (PS)/poly(styrene-alt-maleic anhydride) (PSMA) fibers with diameters ranging from 0.5 to 1.0 m and photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun fibers created clearly defined hydrogel microstructures with incorporated nanofibers. The resultant micropatterned nanofibrous substrates were obtained as freestanding and bidirectionally porous sheets, where most of the nanofibers were inserted through the side walls of the hydrogel microstructures. Because of the protein-repellent nature of PEG hydrogels, IgG was selectively immobilized only within the nanofibrous region, creating an IgG microarray. Due to increased surface area, IgG loading in nanofibrous substrates was about six times greater than on planar substrates, which consequently yielded a higher fluorescence signal and faster reaction rate in immunoassays. The capability of encapsulating enzymes made it possible for PEG hydrogels to be used not only for defining protein micropatterns but also for additional biosensor elements. Based on this result, micropatterned nanofibrous substrates consisting of IgG-immobilized nanofibers and enzyme-entrapping PEG hydrogels were fabricated, and their potential to simultaneously carry out both immunoassays and enzyme-based assays was successfully demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=79952588057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952588057&partnerID=8YFLogxK

U2 - 10.1039/c0jm03881d

DO - 10.1039/c0jm03881d

M3 - Article

VL - 21

SP - 4476

EP - 4483

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 12

ER -