Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration

Y. K. Lee, Y. S. Park, M. C. Kim, Kwangmahn Kim, K. N. Kim, Seongho Choi, C. K. Kim, Hansung Jung, C. K. You, R. Z. LeGeros

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

Numerous techniques have been applied to fabricate three-dimensional scaffolds of high porosity and surface area. In this study, we fabricated three-dimensional macroporous scaffold by polymeric sponge method using calcium phosphate glass in CaO-CaF2-P2O5-MgO-ZnO. Calcium phosphate glass slurry was prepared by suspending the glass powder in water, polyvinyl alcohol, polyethylene glycol and dimethyl formamide. Reticulated polyurethane sponges were used as a template and were coated with the prepared slurry by infiltration technique several times. Calcium phosphate glass slurry was homogenously thick coated when the amounts of calcium phosphate glass powder and polyvinyl alcohol were 67 and 8 wt%, respectively. Addition of 10 wt% dimethyl formamide as a drying control chemical additive into a slurry successively prevented microcrack formation. Sintering at 850°C exhibited dense microstructures as well as entire elimination of organic additives. When the macroporous scaffolds were transplanted subcutaneously in the dorsal surface of mice, the vascularized mesenchymal stem cells were formed in the transplanted scaffolds and the regenerated connective tissue was also formed after 4 weeks. These results of the present study suggested that the fabricated macroporous scaffold is expected to be useful scaffold material for osteogenic tissue development.

Original languageEnglish
Pages (from-to)1079-1082
Number of pages4
JournalKey Engineering Materials
Volume254-256
Publication statusPublished - 2004 Jan 9
EventThe Annual Meeting of the International Society for Ceramics in Medicine - Porto, Portugal
Duration: 2003 Nov 62003 Nov 9

Fingerprint

Calcium phosphate
Scaffolds
Bone
Fabrication
Glass
Polyvinyl Alcohol
phosphorus pentoxide
Polyvinyl alcohols
Dimethylformamide
Powders
Tissue
Polyurethanes
Microcracks
Stem cells
Infiltration
Polyethylene glycols
calcium phosphate
Drying
Sintering
Porosity

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Lee, Y. K., Park, Y. S., Kim, M. C., Kim, K., Kim, K. N., Choi, S., ... LeGeros, R. Z. (2004). Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration. Key Engineering Materials, 254-256, 1079-1082.
Lee, Y. K. ; Park, Y. S. ; Kim, M. C. ; Kim, Kwangmahn ; Kim, K. N. ; Choi, Seongho ; Kim, C. K. ; Jung, Hansung ; You, C. K. ; LeGeros, R. Z. / Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration. In: Key Engineering Materials. 2004 ; Vol. 254-256. pp. 1079-1082.
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abstract = "Numerous techniques have been applied to fabricate three-dimensional scaffolds of high porosity and surface area. In this study, we fabricated three-dimensional macroporous scaffold by polymeric sponge method using calcium phosphate glass in CaO-CaF2-P2O5-MgO-ZnO. Calcium phosphate glass slurry was prepared by suspending the glass powder in water, polyvinyl alcohol, polyethylene glycol and dimethyl formamide. Reticulated polyurethane sponges were used as a template and were coated with the prepared slurry by infiltration technique several times. Calcium phosphate glass slurry was homogenously thick coated when the amounts of calcium phosphate glass powder and polyvinyl alcohol were 67 and 8 wt{\%}, respectively. Addition of 10 wt{\%} dimethyl formamide as a drying control chemical additive into a slurry successively prevented microcrack formation. Sintering at 850°C exhibited dense microstructures as well as entire elimination of organic additives. When the macroporous scaffolds were transplanted subcutaneously in the dorsal surface of mice, the vascularized mesenchymal stem cells were formed in the transplanted scaffolds and the regenerated connective tissue was also formed after 4 weeks. These results of the present study suggested that the fabricated macroporous scaffold is expected to be useful scaffold material for osteogenic tissue development.",
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Lee, YK, Park, YS, Kim, MC, Kim, K, Kim, KN, Choi, S, Kim, CK, Jung, H, You, CK & LeGeros, RZ 2004, 'Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration', Key Engineering Materials, vol. 254-256, pp. 1079-1082.

Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration. / Lee, Y. K.; Park, Y. S.; Kim, M. C.; Kim, Kwangmahn; Kim, K. N.; Choi, Seongho; Kim, C. K.; Jung, Hansung; You, C. K.; LeGeros, R. Z.

In: Key Engineering Materials, Vol. 254-256, 09.01.2004, p. 1079-1082.

Research output: Contribution to journalConference article

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T1 - Fabrication of Macroporous Scaffold Using Calcium Phosphate Glass for Bone Regeneration

AU - Lee, Y. K.

AU - Park, Y. S.

AU - Kim, M. C.

AU - Kim, Kwangmahn

AU - Kim, K. N.

AU - Choi, Seongho

AU - Kim, C. K.

AU - Jung, Hansung

AU - You, C. K.

AU - LeGeros, R. Z.

PY - 2004/1/9

Y1 - 2004/1/9

N2 - Numerous techniques have been applied to fabricate three-dimensional scaffolds of high porosity and surface area. In this study, we fabricated three-dimensional macroporous scaffold by polymeric sponge method using calcium phosphate glass in CaO-CaF2-P2O5-MgO-ZnO. Calcium phosphate glass slurry was prepared by suspending the glass powder in water, polyvinyl alcohol, polyethylene glycol and dimethyl formamide. Reticulated polyurethane sponges were used as a template and were coated with the prepared slurry by infiltration technique several times. Calcium phosphate glass slurry was homogenously thick coated when the amounts of calcium phosphate glass powder and polyvinyl alcohol were 67 and 8 wt%, respectively. Addition of 10 wt% dimethyl formamide as a drying control chemical additive into a slurry successively prevented microcrack formation. Sintering at 850°C exhibited dense microstructures as well as entire elimination of organic additives. When the macroporous scaffolds were transplanted subcutaneously in the dorsal surface of mice, the vascularized mesenchymal stem cells were formed in the transplanted scaffolds and the regenerated connective tissue was also formed after 4 weeks. These results of the present study suggested that the fabricated macroporous scaffold is expected to be useful scaffold material for osteogenic tissue development.

AB - Numerous techniques have been applied to fabricate three-dimensional scaffolds of high porosity and surface area. In this study, we fabricated three-dimensional macroporous scaffold by polymeric sponge method using calcium phosphate glass in CaO-CaF2-P2O5-MgO-ZnO. Calcium phosphate glass slurry was prepared by suspending the glass powder in water, polyvinyl alcohol, polyethylene glycol and dimethyl formamide. Reticulated polyurethane sponges were used as a template and were coated with the prepared slurry by infiltration technique several times. Calcium phosphate glass slurry was homogenously thick coated when the amounts of calcium phosphate glass powder and polyvinyl alcohol were 67 and 8 wt%, respectively. Addition of 10 wt% dimethyl formamide as a drying control chemical additive into a slurry successively prevented microcrack formation. Sintering at 850°C exhibited dense microstructures as well as entire elimination of organic additives. When the macroporous scaffolds were transplanted subcutaneously in the dorsal surface of mice, the vascularized mesenchymal stem cells were formed in the transplanted scaffolds and the regenerated connective tissue was also formed after 4 weeks. These results of the present study suggested that the fabricated macroporous scaffold is expected to be useful scaffold material for osteogenic tissue development.

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