Fabrication of nano-scale liposomes containing doxorubicin using Shirasu porous glass membrane

Taewon Hwang, Tae Joon Park, Won Gun Koh, In Woo Cheong, Sung Wook Choi, Jung Hyun Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Nano-scale liposomes were successfully produced using a Shirasu porous glass (SPG) membrane emulsification technique. Primary liposomes prepared by a film-hydration method were treated using SPG membranes with different pore sizes (2.0, 1.0, 0.7, 0.5, and 0.2μm) for control over the liposome size. The liposome sizes were evaluated using a dynamic light scattering method and their morphologies were observed by optical microscopy and transmission electron microscopy. As the passage number of liposomes through SPG membrane increased, the size and its distribution of the liposomes gradually decreased. A smaller pore size of the SPG membrane and a higher applied pressure resulted in liposomes with a smaller size. After the preparation of nano-scale liposomes containing ammonium sulfate (AS), doxorubicin (DOX) was encapsulated in the liposomes by a remote loading method, where AS served as a precipitant for DOX. The encapsulation efficiency of the DOX was maximized up to 94% when the concentrations of AS and DOX were 250 and 0.045. mM, respectively. We have obtained the release profiles of the liposomes with different sizes. As shown below, liposomes with smaller size exhibited a faster release profile of drug due to the large surface area. These nano-scale liposomes encapsulating an anti-cancer drug can potentially be employed as drug delivery vehicles for intravenous injection.

Original languageEnglish
Pages (from-to)250-255
Number of pages6
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume392
Issue number1
DOIs
Publication statusPublished - 2011 Dec 5

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) grant funded by the Korea government (MEST) through the Active Polymer Center for Pattern Integration (No. R11-2007-050-00000-0 and 2007-0052622), a grant from the Industrial Technology Development program ( K0006005 ) of the Ministry of Knowledge Economy (MKE) of Korea, Nano·Material Technology Development Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education, Science and Technology ( 2001-0019176 ), the Seoul Research and Business Development Program ( 10816 ), the Pioneer Research Center Program though the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (No. 2010-0019308 ), Mid-career Researcher Program through NRF grant funded by the MEST (No. 2007-0052622 ), a grant from the “GRRC” Project of Gyeonggi Provincial Government, Korea, a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (A110416), and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2011-0023064 ).

All Science Journal Classification (ASJC) codes

  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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