Fabrication of photofunctional nanoporous membrane and its photoinactivation effect of vesicular stomatitis virus

Kang Kyun Wang, Bong Jin Kim, Si Hwan Ko, Dong Hoon Choi, Yong Rok Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4- methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.

Original languageEnglish
Article number454507
JournalJournal of Nanomaterials
Volume2012
DOIs
Publication statusPublished - 2012 Jan 1

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Aluminum Oxide
Viruses
Alumina
Membranes
Fabrication
Chemotherapy
Spectroscopy
Silanes
Photosensitizing Agents
Photosensitizers
Porphyrins
Anodic oxidation
Platinum
Aluminum
Field emission
Antiviral Agents
Animals
Substitution reactions
Electron microscopes
Irradiation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

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abstract = "Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4- methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.",
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Fabrication of photofunctional nanoporous membrane and its photoinactivation effect of vesicular stomatitis virus. / Wang, Kang Kyun; Kim, Bong Jin; Ko, Si Hwan; Choi, Dong Hoon; Kim, Yong Rok.

In: Journal of Nanomaterials, Vol. 2012, 454507, 01.01.2012.

Research output: Contribution to journalArticle

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