TY - GEN
T1 - Fabrication of swellable microneedle using methacrylated hyaluronic acid for extraction of biomarkers in ocular fluids
AU - Park, Seung Hyun
AU - Lee, Jiyong
AU - Ryu, Wonhyoung
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Introduction: Interstitial fluid (ISF) in the ocular tissue has many biomarkers from ions to proteins. Since the concentration of biomarkers such as lactate and cytokine increase in the presence of cancer, monitoring the level of biomarkers is important to diagnose and treat the diseases. However, there are limited number of tools to collect disease markers from tissue although there are many analytical methods. Herein this study, we developed swellable microneedle (MN) device which was minimally invasive and pain-free to detect the levels of biomarkers from ISF. First, methacrylated hyaluronic acid (MeHA) were synthesized and we fabricated swellable MeHA MNs by a combination of photo-crosslinking and transfer molding. Then, the mechanical stability was confirmed by ex vivo compression tests and in vitro extraction of model biomarkers from phantom tissue was demonstrated.
AB - Introduction: Interstitial fluid (ISF) in the ocular tissue has many biomarkers from ions to proteins. Since the concentration of biomarkers such as lactate and cytokine increase in the presence of cancer, monitoring the level of biomarkers is important to diagnose and treat the diseases. However, there are limited number of tools to collect disease markers from tissue although there are many analytical methods. Herein this study, we developed swellable microneedle (MN) device which was minimally invasive and pain-free to detect the levels of biomarkers from ISF. First, methacrylated hyaluronic acid (MeHA) were synthesized and we fabricated swellable MeHA MNs by a combination of photo-crosslinking and transfer molding. Then, the mechanical stability was confirmed by ex vivo compression tests and in vitro extraction of model biomarkers from phantom tissue was demonstrated.
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M3 - Conference contribution
AN - SCOPUS:85065427524
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -