Objective: We investigated the association of polymorphisms in FADS genes with polyunsaturated fatty acids (PUFAs) in serum phospholipids, lipid peroxides, and coronary artery disease (CAD) in Koreans. Methods: In this case-control study, CAD patients (n= 756, 40-79 years) and healthy controls (n= 890) were genotyped for rs174537 near FADS1 (FEN1 rs174537G > T), FADS2 (rs174575, rs2727270), and FADS3 (rs1000778). We calculated the odds ratios (ORs) for CAD risk and measured serum PUFA composition and lipid peroxide. Results: Among four SNPs, only rs174537G>T differed in allele frequencies between controls and CAD patients after adjustment for age, BMI, cigarette smoking, alcohol consumption, hypertension, diabetes mellitus, and hyperlipidemia (P=0.017). The minor T allele was associated with a lower risk of CAD [OR 0.75 (95%CI 0.61-0.92), P=0.006] after adjustment. rs174537T carriers had a significantly higher proportion of linoleic acid (LA, 18:2ω6), lower arachidonic acid (AA, 20:4ω6), and lower ratios of AA/dihomo-γ-linolenic acid (DGLA, 20:3ω6) and AA/LA than G/G subjects in both control and CAD groups. In the control group, 174537T carriers had significantly lower levels of total- and LDL-cholesterol, malondialdehyde, and ox-LDL. In CAD patients, rs174537T carriers showed a larger LDL particle size than G/G subjects. The proportion of AA in serum phospholipids positively correlated with LDL-cholesterol, ox-LDL, and malondialdehyde in controls and with 8-epi-prostaglandin F 2α in both control and CAD groups. Conclusion: The rs174537T is associated with a lower proportion of AA in serum phospholipids and reduced CAD risk, in association with reduced total- and LDL-cholesterol and lipid peroxides.
|Number of pages||7|
|Publication status||Published - 2011 Jan|
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation, Ministry of Education, Science and Technology (Mid-career Researcher Program: 2010-0015017 , M10642120002-06N4212-00210 and C00048 ) and the Korea Health 21 R&D Projects, Ministry of Health & Welfare ( A000385 ), Republic of Korea, and in part by the Yonsei University Research Fund of 2010 , Seoul, Republic of Korea.
All Science Journal Classification (ASJC) codes
- Cardiology and Cardiovascular Medicine