FAK mediates signal crosstalk between type II collagen and TGF-beta 1 cascades in chondrocytic cells

Min Sung Park, Yun Hee Kim, jinwoo lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The purpose of this study was to evaluate the mechanism of crosstalk between the type II collagen and TGF-β1 signaling pathways in chondrocytic cells. Articular chondrocytes, isolated from porcine knee cartilage, and the SW1353 cell line were cultured on either type II collagen-coated or -uncoated plates in the presence or absence of TGF-β1. Expression of pSMAD 2, pSMAD 3, pFAK Y397 and pFAK Y925 in articular chondrocytes and the SW1353 cell line was analyzed by immunoblotting. Cell proliferation rates and glycosaminoglycan (GAG) content was determined after treatment with type II collagen or/and TGF-β1. For inhibition study, human FAK-specific RNA small interference (siFAK) in SW1353 cell line was performed. In this study, expression of pSMAD 2, pSMAD 3, pFAK Y397 and pFAK Y925 were synergistically increased by co-treatment with type II collagen and TGF-β1 in articular chondrocytes. The proliferation of porcine articular chondrocytes and GAG secretion in SW1353 cells were synergistically increased by co-stimulation with type II collagen and TGF-β1. Synergistically increased expression and nuclear translocation of pSMAD 2 and pSMAD 3 and GAG secretion of SW1353 cells were significantly inhibited by siFAK transfection. Therefore, we suggest that FAK-SMAD 2/3 mediates signal crosstalk between type II collagen and TGF-β1 and regulates GAG secretion in chondrocytic cells.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalMatrix Biology
Volume29
Issue number2
DOIs
Publication statusPublished - 2010 Mar 1

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Transforming Growth Factor beta1
Collagen Type II
Chondrocytes
Glycosaminoglycans
Joints
Cell Line
Swine
RNA Interference
Immunoblotting
Cartilage
Transfection
Knee
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

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abstract = "The purpose of this study was to evaluate the mechanism of crosstalk between the type II collagen and TGF-β1 signaling pathways in chondrocytic cells. Articular chondrocytes, isolated from porcine knee cartilage, and the SW1353 cell line were cultured on either type II collagen-coated or -uncoated plates in the presence or absence of TGF-β1. Expression of pSMAD 2, pSMAD 3, pFAK Y397 and pFAK Y925 in articular chondrocytes and the SW1353 cell line was analyzed by immunoblotting. Cell proliferation rates and glycosaminoglycan (GAG) content was determined after treatment with type II collagen or/and TGF-β1. For inhibition study, human FAK-specific RNA small interference (siFAK) in SW1353 cell line was performed. In this study, expression of pSMAD 2, pSMAD 3, pFAK Y397 and pFAK Y925 were synergistically increased by co-treatment with type II collagen and TGF-β1 in articular chondrocytes. The proliferation of porcine articular chondrocytes and GAG secretion in SW1353 cells were synergistically increased by co-stimulation with type II collagen and TGF-β1. Synergistically increased expression and nuclear translocation of pSMAD 2 and pSMAD 3 and GAG secretion of SW1353 cells were significantly inhibited by siFAK transfection. Therefore, we suggest that FAK-SMAD 2/3 mediates signal crosstalk between type II collagen and TGF-β1 and regulates GAG secretion in chondrocytic cells.",
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FAK mediates signal crosstalk between type II collagen and TGF-beta 1 cascades in chondrocytic cells. / Park, Min Sung; Kim, Yun Hee; lee, jinwoo.

In: Matrix Biology, Vol. 29, No. 2, 01.03.2010, p. 135-142.

Research output: Contribution to journalArticle

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