FasL Is Required for Osseous Healing in Extraction Sockets in Mice

Karol Alí Apaza Alccayhuaman, Patrick Heimel, Jung Seok Lee, Stefan Tangl, Franz J. Strauss, Alexandra Stähli, Eva Matalová, Reinhard Gruber

Research output: Contribution to journalArticlepeer-review

Abstract

Fas ligand (FasL) is a member of the tumor necrosis factor (TNF) superfamily involved in the activation of apoptosis. Assuming that apoptosis is initiated after tooth extraction it is reasonable to suggest that FasL may play a pivotal role in the healing of extraction sockets. Herein, we tested the hypothesis of whether the lack of FasL impairs the healing of extraction sockets. To this end, we extracted upper right incisors of FasL knockout (KO) mice and their wildtype (WT) littermates. After a healing period of two weeks, bone volume over total volume (BV/TV) via µCT and descriptive histological analyses were performed. µCT revealed that BV/TV in the coronal region of the socket amounted to 39.4% in WT and 21.8% in KO, with a significant difference between the groups (p=0.002). Likewise, in the middle region of the socket, BV/TV amounted to 50.3% in WT and 40.8% in KO (p<0.001). In the apical part, however, no difference was noticed. Consistently, WT mice displayed a significantly higher median trabecular thickness and a lower trabecular separation when compared to the KO group at the coronal and central region of the socket. There was the overall tendency that in both, female and male mice, FasL affects bone regeneration. Taken together, these findings suggest that FasL deficiency may reduce bone regeneration during the healing process of extraction sockets.

Original languageEnglish
Article number678873
JournalFrontiers in Immunology
Volume12
DOIs
Publication statusPublished - 2021 May 31

Bibliographical note

Funding Information:
This research project is funded by a grant from the Austrian Science Fund (FWF) (4072‐B28) joint with the Czech Science Foundation (GACR) (19-29667L). KAAA is supported by an Osteology Research Scholarship.

Publisher Copyright:
© Copyright © 2021 Apaza Alccayhuaman, Heimel, Lee, Tangl, Strauss, Stähli, Matalová and Gruber.

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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