Fast cell immobilization by using non-immunological method for cell based biosensor

Jihwang Park, Michael Müller, Eunji Jang, Jungtae Kim, Lan Young Hong, Dong Pyo Kim, Helmut Seidel, Won Gun Koh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non-immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5×106, 1.0×106 or 2.0×106 cells/ml in micro wells with 10μm depth and 25μm, 50μm and 100μm size respectively was performed. Different tendencies of cell adhesion between 0.5×106 cells/ml and 1.0×10 6 cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.

Original languageEnglish
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages1932-1935
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: 2010 Nov 12010 Nov 4

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period10/11/110/11/4

Fingerprint

Cell immobilization
Cell adhesion
Biosensors
Inlet flow
Experiments

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Park, J., Müller, M., Jang, E., Kim, J., Hong, L. Y., Kim, D. P., ... Koh, W. G. (2010). Fast cell immobilization by using non-immunological method for cell based biosensor. In IEEE Sensors 2010 Conference, SENSORS 2010 (pp. 1932-1935). [5689990] https://doi.org/10.1109/ICSENS.2010.5689990
Park, Jihwang ; Müller, Michael ; Jang, Eunji ; Kim, Jungtae ; Hong, Lan Young ; Kim, Dong Pyo ; Seidel, Helmut ; Koh, Won Gun. / Fast cell immobilization by using non-immunological method for cell based biosensor. IEEE Sensors 2010 Conference, SENSORS 2010. 2010. pp. 1932-1935
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abstract = "This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non-immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5×106, 1.0×106 or 2.0×106 cells/ml in micro wells with 10μm depth and 25μm, 50μm and 100μm size respectively was performed. Different tendencies of cell adhesion between 0.5×106 cells/ml and 1.0×10 6 cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.",
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Park, J, Müller, M, Jang, E, Kim, J, Hong, LY, Kim, DP, Seidel, H & Koh, WG 2010, Fast cell immobilization by using non-immunological method for cell based biosensor. in IEEE Sensors 2010 Conference, SENSORS 2010., 5689990, pp. 1932-1935, 9th IEEE Sensors Conference 2010, SENSORS 2010, Waikoloa, HI, United States, 10/11/1. https://doi.org/10.1109/ICSENS.2010.5689990

Fast cell immobilization by using non-immunological method for cell based biosensor. / Park, Jihwang; Müller, Michael; Jang, Eunji; Kim, Jungtae; Hong, Lan Young; Kim, Dong Pyo; Seidel, Helmut; Koh, Won Gun.

IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 1932-1935 5689990.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Park J, Müller M, Jang E, Kim J, Hong LY, Kim DP et al. Fast cell immobilization by using non-immunological method for cell based biosensor. In IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 1932-1935. 5689990 https://doi.org/10.1109/ICSENS.2010.5689990