Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

Jiyong Cheong; Hojeong Yu; Chang Yeol Lee; Jung uk Lee; Hyun Jung Choi; Jae Hyun Lee; Hakho Lee; Jinwoo Cheon

doi:10.1038/s41551-020-00654-0

Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

Jiyong Cheong, Hojeong Yu, Chang Yeol Lee, Jung uk Lee, Hyun Jung Choi, Jae Hyun Lee, Hakho Lee, Jinwoo Cheon

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT–qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1–2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT–qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.

Original language	English
Pages (from-to)	1159-1167
Number of pages	9
Journal	Nature biomedical engineering
Volume	4
Issue number	12
DOIs	https://doi.org/10.1038/s41551-020-00654-0
Publication status	Published - 2020 Dec

Bibliographical note

Funding Information:
We thank S.-Y. Cheon for graphic designs and illustrations of figures and POC devices. This work was supported by the Institute for Basic Science (IBS-R026-D1). H.L. was supported in part by US NIH grants R01CA229777, R21DA049577 and U01CA233360, US DOD-W81XWH1910199 and DOD-W81XWH1910194 and the MGH Scholar Fund.

Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
Computer Science Applications

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abstract = "The diagnosis of severe acute respiratory syndrome 2 (SARS-CoV-2) infection by quantitative PCR with reverse transcription (RT–qPCR) typically involves bulky instrumentation in centralized laboratories and an assay time of 1–2 h. Here, we show that SARS-CoV-2 RNA can be detected in 17 min via a portable device integrating reverse transcription, fast thermocycling (via plasmonic heating through magneto-plasmonic nanoparticles) and in situ fluorescence detection following magnetic clearance of the nanoparticles. The device correctly classified all nasopharyngeal, oropharyngeal and sputum samples from 75 patients with COVID-19 and 75 healthy controls, with good concordance in fluorescence intensity with standard RT–qPCR (Pearson coefficients > 0.7 for the N1, N2 and RPP30 genes). Fast, portable and automated nucleic acid detection should facilitate testing at the point of care.",

author = "Jiyong Cheong and Hojeong Yu and Lee, {Chang Yeol} and Lee, {Jung uk} and Choi, {Hyun Jung} and Lee, {Jae Hyun} and Hakho Lee and Jinwoo Cheon",

note = "Funding Information: We thank S.-Y. Cheon for graphic designs and illustrations of figures and POC devices. This work was supported by the Institute for Basic Science (IBS-R026-D1). H.L. was supported in part by US NIH grants R01CA229777, R21DA049577 and U01CA233360, US DOD-W81XWH1910199 and DOD-W81XWH1910194 and the MGH Scholar Fund. Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature Limited.",

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AU - Choi, Hyun Jung

AU - Lee, Jae Hyun

AU - Lee, Hakho

AU - Cheon, Jinwoo

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Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device

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