Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation

Periyasamy Sivagurunathan, Gopalakrishnan Kumar, Jeong Hoon Park, Jong Hun Park, Hee Deung Park, Jeong Jun Yoon, Sang Hyoun Kim

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

This research investigated the suitability of enriched mixed cultures (EMC) for anaerobic hydrogen fermentation in continuous operation. EMC was prepared after four successive transfers in PYG (peptone, yeast extract and galactose) medium in batch cultivation. The peak hydrogen production rate (HPR) and hydrogen yield (HY) of 770 ± 10 mL H2/L-d and 1.05 ± 0.06 mol H2/mol galactoseadded, were attained respectively. There forward a continuously stirred tank reactor (CSTR) has been operated with the substrate concentration of 15 g/L at a hydraulic retention time (HRT) of 12 h for more than 15 days by using EMC. The performance showed that HPR and HY were fluctuated significantly during the operation and the average values were 1710 ± 250 mL H2/L-d and 0.82 ± 0.12 mol H2/mol galactoseadded, respectively. The soluble metabolic products analysis revealed that butyrate, lactate and acetate were the dominant metabolic products with less quantity of propionic and formic acids. The microbial community structure has been determined by next generation DNA sequencing technique and revealed Clostridium sp. was the dominant microbial consortium during repeated batch transfer, whereas Sporolactobacillus sp. was the major population in continuous operation. This study demonstrates that operational mode (batch and continuous) significantly influence the microbial diversity and hydrogen production, and EMC obtained by repeated process may not be suitable for continuous hydrogen fermentation.

Original languageEnglish
Pages (from-to)4393-4403
Number of pages11
JournalInternational Journal of Hydrogen Energy
Volume41
Issue number7
DOIs
Publication statusPublished - 2016 Feb 23

Fingerprint

fermentation
Fermentation
hydrogen production
Hydrogen production
Hydrogen
hydrogen
Clostridium
galactose
propionic acid
Propionic acid
lactates
sequencing
Formic acid
yeast
formic acid
products
hydraulics
Yeast
acetates
DNA

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Sivagurunathan, Periyasamy ; Kumar, Gopalakrishnan ; Park, Jeong Hoon ; Park, Jong Hun ; Park, Hee Deung ; Yoon, Jeong Jun ; Kim, Sang Hyoun. / Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation. In: International Journal of Hydrogen Energy. 2016 ; Vol. 41, No. 7. pp. 4393-4403.
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Feasibility of enriched mixed cultures obtained by repeated batch transfer in continuous hydrogen fermentation. / Sivagurunathan, Periyasamy; Kumar, Gopalakrishnan; Park, Jeong Hoon; Park, Jong Hun; Park, Hee Deung; Yoon, Jeong Jun; Kim, Sang Hyoun.

In: International Journal of Hydrogen Energy, Vol. 41, No. 7, 23.02.2016, p. 4393-4403.

Research output: Contribution to journalArticle

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