Abstract
We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.
| Original language | English |
|---|---|
| Title of host publication | Multimodal Biomedical Imaging III |
| DOIs | |
| Publication status | Published - 2008 Apr 21 |
| Event | Multimodal Biomedical Imaging III - San Jose, CA, United States Duration: 2008 Jan 19 → 2008 Jan 21 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 6850 |
| ISSN (Print) | 1605-7422 |
Other
| Other | Multimodal Biomedical Imaging III |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 08/1/19 → 08/1/21 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
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Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. / Kim, Kyujung; Cho, Eun Jin; Huh, Yong Min; Kim, Donghyun.
Multimodal Biomedical Imaging III. 2008. 68500R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films
AU - Kim, Kyujung
AU - Cho, Eun Jin
AU - Huh, Yong Min
AU - Kim, Donghyun
PY - 2008/4/21
Y1 - 2008/4/21
N2 - We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.
AB - We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.
UR - http://www.scopus.com/inward/record.url?scp=42149175036&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149175036&partnerID=8YFLogxK
U2 - 10.1117/12.765621
DO - 10.1117/12.765621
M3 - Conference contribution
AN - SCOPUS:42149175036
SN - 9780819470256
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Multimodal Biomedical Imaging III
ER -