Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films

Kyujung Kim, Eun Jin Cho, Yong Min Huh, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging III
DOIs
Publication statusPublished - 2008 Apr 21
EventMultimodal Biomedical Imaging III - San Jose, CA, United States
Duration: 2008 Jan 192008 Jan 21

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6850
ISSN (Print)1605-7422

Other

OtherMultimodal Biomedical Imaging III
CountryUnited States
CitySan Jose, CA
Period08/1/1908/1/21

Fingerprint

Quantum Dots
Dielectric films
Optical Imaging
Feasibility Studies
Microspheres
Fluorescence Microscopy
Fluorescence
Cell Membrane
Imaging techniques
fluorescence
augmentation
Fluorescence microscopy
Incidence
Cell membranes
Prisms
Semiconductor quantum dots
Polarization
Thin films
prisms
Substrates

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, K., Cho, E. J., Huh, Y. M., & Kim, D. (2008). Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. In Multimodal Biomedical Imaging III [68500R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850). https://doi.org/10.1117/12.765621
Kim, Kyujung ; Cho, Eun Jin ; Huh, Yong Min ; Kim, Donghyun. / Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. Multimodal Biomedical Imaging III. 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Kim, K, Cho, EJ, Huh, YM & Kim, D 2008, Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. in Multimodal Biomedical Imaging III., 68500R, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6850, Multimodal Biomedical Imaging III, San Jose, CA, United States, 08/1/19. https://doi.org/10.1117/12.765621

Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. / Kim, Kyujung; Cho, Eun Jin; Huh, Yong Min; Kim, Donghyun.

Multimodal Biomedical Imaging III. 2008. 68500R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kim K, Cho EJ, Huh YM, Kim D. Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. In Multimodal Biomedical Imaging III. 2008. 68500R. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.765621