@inproceedings{b0291ce3095040a0938c620bdb655b99,
title = "Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films",
abstract = "We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8{\^A} for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.",
author = "Kyujung Kim and Cho, {Eun Jin} and Huh, {Yong Min} and Donghyun Kim",
year = "2008",
doi = "10.1117/12.765621",
language = "English",
isbn = "9780819470256",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Multimodal Biomedical Imaging III",
note = "Multimodal Biomedical Imaging III ; Conference date: 19-01-2008 Through 21-01-2008",
}