Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films

Kyujung Kim, Eun Jin Cho, Yong Min Huh, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging III
DOIs
Publication statusPublished - 2008 Apr 21
EventMultimodal Biomedical Imaging III - San Jose, CA, United States
Duration: 2008 Jan 192008 Jan 21

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6850
ISSN (Print)1605-7422

Other

OtherMultimodal Biomedical Imaging III
CountryUnited States
CitySan Jose, CA
Period08/1/1908/1/21

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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    Kim, K., Cho, E. J., Huh, Y. M., & Kim, D. (2008). Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. In Multimodal Biomedical Imaging III [68500R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850). https://doi.org/10.1117/12.765621