Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films

Kyujung Kim, Eun Jin Cho, Yong Min Huh, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.

Original languageEnglish
Title of host publicationMultimodal Biomedical Imaging III
Publication statusPublished - 2008
EventMultimodal Biomedical Imaging III - San Jose, CA, United States
Duration: 2008 Jan 192008 Jan 21

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherMultimodal Biomedical Imaging III
Country/TerritoryUnited States
CitySan Jose, CA

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


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