Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films

Kyujung Kim; Eun Jin Cho; Yong Min Huh; Donghyun Kim

doi:10.1117/12.765621

Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films

Kyujung Kim, Eun Jin Cho, Yong Min Huh, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.

Original language	English
Title of host publication	Multimodal Biomedical Imaging III
DOIs	https://doi.org/10.1117/12.765621
Publication status	Published - 2008 Apr 21
Event	Multimodal Biomedical Imaging III - San Jose, CA, United States Duration: 2008 Jan 19 → 2008 Jan 21

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6850
ISSN (Print)	1605-7422

Other

Other	Multimodal Biomedical Imaging III
Country	United States
City	San Jose, CA
Period	08/1/19 → 08/1/21

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All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Cite this

Kim, K., Cho, E. J., Huh, Y. M., & Kim, D. (2008). Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. In Multimodal Biomedical Imaging III [68500R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850). https://doi.org/10.1117/12.765621

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title = "Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films",

abstract = "We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8{\^A} for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.",

author = "Kyujung Kim and Cho, {Eun Jin} and Huh, {Yong Min} and Donghyun Kim",

year = "2008",

month = apr,

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doi = "10.1117/12.765621",

language = "English",

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Kim, K, Cho, EJ, Huh, YM & Kim, D 2008, Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. in Multimodal Biomedical Imaging III., 68500R, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6850, Multimodal Biomedical Imaging III, San Jose, CA, United States, 08/1/19. https://doi.org/10.1117/12.765621

Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. / Kim, Kyujung; Cho, Eun Jin; Huh, Yong Min ; Kim, Donghyun.

Multimodal Biomedical Imaging III. 2008. 68500R (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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N2 - We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.

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10.1117/12.765621