Abstract
We present the sensitivity improvement of total internal reflection fluorescence microscopy for imaging intracellular molecular movements near cell membranes. We investigated employing dielectric films on a prism substrate for the enhancement of fluorescence emission intensity. A two-layer dielectric thin film structure using Al2O3 and SiO2 was designed and fabricated to provide the maximal field enhancement for 442 nm excitation at a reasonable angle of incidence (). The field enhancement achieved by the design was 8.5 at = 53.8Â for TE polarization and confirmed experimentally using microbeads. Preliminary results in live cell imaging were obtained using quantum dots.
| Original language | English |
|---|---|
| Title of host publication | Multimodal Biomedical Imaging III |
| DOIs | |
| Publication status | Published - 2008 Apr 21 |
| Event | Multimodal Biomedical Imaging III - San Jose, CA, United States Duration: 2008 Jan 19 → 2008 Jan 21 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 6850 |
| ISSN (Print) | 1605-7422 |
Other
| Other | Multimodal Biomedical Imaging III |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 08/1/19 → 08/1/21 |
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
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Kim, K., Cho, E. J., Huh, Y. M., & Kim, D. (2008). Feasibility study of enhanced total internal reflection fluorescence imaging using dielectric films. In Multimodal Biomedical Imaging III [68500R] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6850). https://doi.org/10.1117/12.765621