Femtosecond cellular transfection using a nondiffracting light beam

X. Tsampoula; V. Garćs-Chávez; M. Comrie; D. J. Stevenson; B. Agate; C. T.A. Brown; F. Gunn-Moore; K. Dholakia

doi:10.1063/1.2766835

Femtosecond cellular transfection using a nondiffracting light beam

X. Tsampoula, V. Garćs-Chávez, M. Comrie, D. J. Stevenson, B. Agate, C. T.A. Brown, F. Gunn-Moore, K. Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

140 Citations (Scopus)

Abstract

The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam.

Original language	English
Article number	053902
Journal	Applied Physics Letters
Volume	91
Issue number	5
DOIs	https://doi.org/10.1063/1.2766835
Publication status	Published - 2007

Bibliographical note

Funding Information:
The authors thank R. F. Marchington and P. Fischer for useful discussions and experimental assistance. The authors also thank the UK Engineering and Physical Sciences Research Council and the Scottish Higher Education Funding Council for funding. Two of the authors (F. G.-M. and K.D.) contributed equally to this work.

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.2766835

Cite this

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AU - Tsampoula, X.

AU - Garćs-Chávez, V.

AU - Comrie, M.

AU - Stevenson, D. J.

AU - Agate, B.

AU - Brown, C. T.A.

AU - Gunn-Moore, F.

AU - Dholakia, K.

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AB - The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam.

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Femtosecond cellular transfection using a nondiffracting light beam

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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