Femtosecond cellular transfection using a nondiffracting light beam

X. Tsampoula, V. Garćs-Chávez, M. Comrie, D. J. Stevenson, B. Agate, C. T.A. Brown, F. Gunn-Moore, K. Dholakia

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129 Citations (Scopus)


The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam.

Original languageEnglish
Article number053902
JournalApplied Physics Letters
Issue number5
Publication statusPublished - 2007 Aug 10

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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  • Cite this

    Tsampoula, X., Garćs-Chávez, V., Comrie, M., Stevenson, D. J., Agate, B., Brown, C. T. A., Gunn-Moore, F., & Dholakia, K. (2007). Femtosecond cellular transfection using a nondiffracting light beam. Applied Physics Letters, 91(5), [053902]. https://doi.org/10.1063/1.2766835