Femtosecond optical injection of intact plant cells using a reconfigurable platform

Claire A. Mitchell, Stefan Kalies, Tomas Cizmar, Nicola Bellini, Anisha Kubasik-Thayil, Alexander Heisterkamp, Lesley Torrance, Alison G. Roberts, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.

Original languageEnglish
Title of host publicationFrontiers in Ultrafast Optics
Subtitle of host publicationBiomedical, Scientific, and Industrial Applications XIV
PublisherSPIE
ISBN (Print)9780819498854
DOIs
Publication statusPublished - 2014 Jan 1
EventFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - San Francisco, CA, United States
Duration: 2014 Feb 22014 Feb 5

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8972
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/5

Fingerprint

Optical Injection
Femtosecond
platforms
injection
Dispersion compensation
Molecules
Gaussian beams
Tobacco
Lasers
delivery
Cell
pulse duration
cells
Pulsed lasers
ultrashort pulsed lasers
Optical systems
tobacco
Laser pulses
Suspensions
Cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Mitchell, C. A., Kalies, S., Cizmar, T., Bellini, N., Kubasik-Thayil, A., Heisterkamp, A., ... Dholakia, K. (2014). Femtosecond optical injection of intact plant cells using a reconfigurable platform. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV [89720C] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8972). SPIE. https://doi.org/10.1117/12.2037784
Mitchell, Claire A. ; Kalies, Stefan ; Cizmar, Tomas ; Bellini, Nicola ; Kubasik-Thayil, Anisha ; Heisterkamp, Alexander ; Torrance, Lesley ; Roberts, Alison G. ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Femtosecond optical injection of intact plant cells using a reconfigurable platform. Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. SPIE, 2014. (Proceedings of SPIE - The International Society for Optical Engineering).
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abstract = "The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.",
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Mitchell, CA, Kalies, S, Cizmar, T, Bellini, N, Kubasik-Thayil, A, Heisterkamp, A, Torrance, L, Roberts, AG, Gunn-Moore, FJ & Dholakia, K 2014, Femtosecond optical injection of intact plant cells using a reconfigurable platform. in Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV., 89720C, Proceedings of SPIE - The International Society for Optical Engineering, vol. 8972, SPIE, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, San Francisco, CA, United States, 14/2/2. https://doi.org/10.1117/12.2037784

Femtosecond optical injection of intact plant cells using a reconfigurable platform. / Mitchell, Claire A.; Kalies, Stefan; Cizmar, Tomas; Bellini, Nicola; Kubasik-Thayil, Anisha; Heisterkamp, Alexander; Torrance, Lesley; Roberts, Alison G.; Gunn-Moore, Frank J.; Dholakia, Kishan.

Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. SPIE, 2014. 89720C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8972).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Mitchell CA, Kalies S, Cizmar T, Bellini N, Kubasik-Thayil A, Heisterkamp A et al. Femtosecond optical injection of intact plant cells using a reconfigurable platform. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. SPIE. 2014. 89720C. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2037784