Femtosecond optical injection of intact plant cells using a reconfigurable platform

Claire A. Mitchell, Stefan Kalies, Tomas Cizmar, Nicola Bellini, Anisha Kubasik-Thayil, Alexander Heisterkamp, Lesley Torrance, Alison G. Roberts, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output.

Original languageEnglish
Title of host publicationFrontiers in Ultrafast Optics
Subtitle of host publicationBiomedical, Scientific, and Industrial Applications XIV
PublisherSPIE
ISBN (Print)9780819498854
DOIs
Publication statusPublished - 2014 Jan 1
EventFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - San Francisco, CA, United States
Duration: 2014 Feb 22014 Feb 5

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8972
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
CountryUnited States
CitySan Francisco, CA
Period14/2/214/2/5

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Mitchell, C. A., Kalies, S., Cizmar, T., Bellini, N., Kubasik-Thayil, A., Heisterkamp, A., Torrance, L., Roberts, A. G., Gunn-Moore, F. J., & Dholakia, K. (2014). Femtosecond optical injection of intact plant cells using a reconfigurable platform. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV [89720C] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8972). SPIE. https://doi.org/10.1117/12.2037784