Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells

M. L. Torres-Mapa, J. Gardner, H. Bradburn, J. King, K. Dholakia, F. Gunn-Moore

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We demonstrate the use of femtosecond optical transfection for the genetic manipulation of human embryonic stem cells. Using a system with an SLM combined with a scanning mirror allows poration of both single-cell and colony-formed human embryonic stem cells in a rapid and targeted manner. In this work, we show successful transfection of plasmid DNA tagged with fluorescent reporters into human embryonic stem cells using three doses of focused femtosecond laser. A significant number of transfected cells retained their undifferentiated morphological feature of large nucleus with high nucleus to cytoplasmic ratio, 48h after photoporation. Furthermore, DNA constructs driven by different types of promoters were also successfully transfected into human embryonic stem cells using this technique.

Original languageEnglish
Title of host publicationFrontiers in Ultrafast Optics
Subtitle of host publicationBiomedical, Scientific, and Industrial Applications XIII
DOIs
Publication statusPublished - 2013 May 29
EventFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII - San Francisco, CA, United States
Duration: 2013 Feb 32013 Feb 5

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8611
ISSN (Print)0277-786X

Conference

ConferenceFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII
CountryUnited States
CitySan Francisco, CA
Period13/2/313/2/5

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Torres-Mapa, M. L., Gardner, J., Bradburn, H., King, J., Dholakia, K., & Gunn-Moore, F. (2013). Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII [861104] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8611). https://doi.org/10.1117/12.2003739