Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells

M. L. Torres-Mapa, J. Gardner, H. Bradburn, J. King, K. Dholakia, F. Gunn-Moore

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We demonstrate the use of femtosecond optical transfection for the genetic manipulation of human embryonic stem cells. Using a system with an SLM combined with a scanning mirror allows poration of both single-cell and colony-formed human embryonic stem cells in a rapid and targeted manner. In this work, we show successful transfection of plasmid DNA tagged with fluorescent reporters into human embryonic stem cells using three doses of focused femtosecond laser. A significant number of transfected cells retained their undifferentiated morphological feature of large nucleus with high nucleus to cytoplasmic ratio, 48h after photoporation. Furthermore, DNA constructs driven by different types of promoters were also successfully transfected into human embryonic stem cells using this technique.

Original languageEnglish
Title of host publicationFrontiers in Ultrafast Optics
Subtitle of host publicationBiomedical, Scientific, and Industrial Applications XIII
DOIs
Publication statusPublished - 2013 May 29
EventFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII - San Francisco, CA, United States
Duration: 2013 Feb 32013 Feb 5

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8611
ISSN (Print)0277-786X

Conference

ConferenceFrontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII
CountryUnited States
CitySan Francisco, CA
Period13/2/313/2/5

Fingerprint

Stem Cells
Femtosecond
stem cells
Stem cells
Manipulation
manipulators
Nucleus
DNA
deoxyribonucleic acid
nuclei
plasmids
Femtosecond Laser
Cell
Ultrashort pulses
cells
Promoter
Scanning
Dose
Mirror
Mirrors

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Torres-Mapa, M. L., Gardner, J., Bradburn, H., King, J., Dholakia, K., & Gunn-Moore, F. (2013). Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII [861104] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8611). https://doi.org/10.1117/12.2003739
Torres-Mapa, M. L. ; Gardner, J. ; Bradburn, H. ; King, J. ; Dholakia, K. ; Gunn-Moore, F. / Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII. 2013. (Proceedings of SPIE - The International Society for Optical Engineering).
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Torres-Mapa, ML, Gardner, J, Bradburn, H, King, J, Dholakia, K & Gunn-Moore, F 2013, Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. in Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII., 861104, Proceedings of SPIE - The International Society for Optical Engineering, vol. 8611, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII, San Francisco, CA, United States, 13/2/3. https://doi.org/10.1117/12.2003739

Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. / Torres-Mapa, M. L.; Gardner, J.; Bradburn, H.; King, J.; Dholakia, K.; Gunn-Moore, F.

Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII. 2013. 861104 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8611).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Torres-Mapa ML, Gardner J, Bradburn H, King J, Dholakia K, Gunn-Moore F. Femtosecond optical transfection as a tool for genetic manipulation of human embryonic stem cells. In Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIII. 2013. 861104. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.2003739