TY - JOUR
T1 - Femtosecond optical transfection of cells:viability and efficiency
AU - Stevenson, D.
AU - Agate, B.
AU - Tsampoula, X.
AU - Fischer, P.
AU - Brown, C. T.A.
AU - Sibbett, W.
AU - Riches, A.
AU - Gunn-Moore, F.
AU - Dholakia, K.
PY - 2006
Y1 - 2006
N2 - Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.
AB - Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.
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U2 - 10.1364/OE.14.007125
DO - 10.1364/OE.14.007125
M3 - Article
C2 - 19529083
AN - SCOPUS:33746924833
VL - 14
SP - 7125
EP - 7133
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 16
ER -