Femtosecond optical transfection of cells:viability and efficiency

D. Stevenson, B. Agate, X. Tsampoula, P. Fischer, C. T.A. Brown, W. Sibbett, A. Riches, F. Gunn-Moore, K. Dholakia

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.

Original languageEnglish
Pages (from-to)7125-7133
Number of pages9
JournalOptics Express
Volume14
Issue number16
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

viability
cells
lasers
ovaries
hamsters
macromolecules
fluence
sapphire
titanium

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Stevenson, D., Agate, B., Tsampoula, X., Fischer, P., Brown, C. T. A., Sibbett, W., ... Dholakia, K. (2006). Femtosecond optical transfection of cells:viability and efficiency. Optics Express, 14(16), 7125-7133. https://doi.org/10.1364/OE.14.007125
Stevenson, D. ; Agate, B. ; Tsampoula, X. ; Fischer, P. ; Brown, C. T.A. ; Sibbett, W. ; Riches, A. ; Gunn-Moore, F. ; Dholakia, K. / Femtosecond optical transfection of cells:viability and efficiency. In: Optics Express. 2006 ; Vol. 14, No. 16. pp. 7125-7133.
@article{20f52a0761b64edcbd96b1fd1276381c,
title = "Femtosecond optical transfection of cells:viability and efficiency",
abstract = "Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10{\%}. Contrary to recent literature, in which 100{\%} efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.",
author = "D. Stevenson and B. Agate and X. Tsampoula and P. Fischer and Brown, {C. T.A.} and W. Sibbett and A. Riches and F. Gunn-Moore and K. Dholakia",
year = "2006",
month = "1",
day = "1",
doi = "10.1364/OE.14.007125",
language = "English",
volume = "14",
pages = "7125--7133",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "16",

}

Stevenson, D, Agate, B, Tsampoula, X, Fischer, P, Brown, CTA, Sibbett, W, Riches, A, Gunn-Moore, F & Dholakia, K 2006, 'Femtosecond optical transfection of cells:viability and efficiency', Optics Express, vol. 14, no. 16, pp. 7125-7133. https://doi.org/10.1364/OE.14.007125

Femtosecond optical transfection of cells:viability and efficiency. / Stevenson, D.; Agate, B.; Tsampoula, X.; Fischer, P.; Brown, C. T.A.; Sibbett, W.; Riches, A.; Gunn-Moore, F.; Dholakia, K.

In: Optics Express, Vol. 14, No. 16, 01.01.2006, p. 7125-7133.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Femtosecond optical transfection of cells:viability and efficiency

AU - Stevenson, D.

AU - Agate, B.

AU - Tsampoula, X.

AU - Fischer, P.

AU - Brown, C. T.A.

AU - Sibbett, W.

AU - Riches, A.

AU - Gunn-Moore, F.

AU - Dholakia, K.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.

AB - Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 μJ/cm2, have found an average transfection efficiency of 50 ± 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.

UR - http://www.scopus.com/inward/record.url?scp=33746924833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746924833&partnerID=8YFLogxK

U2 - 10.1364/OE.14.007125

DO - 10.1364/OE.14.007125

M3 - Article

C2 - 19529083

AN - SCOPUS:33746924833

VL - 14

SP - 7125

EP - 7133

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 16

ER -

Stevenson D, Agate B, Tsampoula X, Fischer P, Brown CTA, Sibbett W et al. Femtosecond optical transfection of cells:viability and efficiency. Optics Express. 2006 Jan 1;14(16):7125-7133. https://doi.org/10.1364/OE.14.007125