Fermentation-mediated enhancement of Ginseng’s anti-allergic activity against ige-mediated passive cutaneous anaphylaxis in vivo and in vitro

Seon Weon Hwang, Xiao Sun, Jun Hyuk Han, Tae Yeon Kim, Sushruta Koppula, Tae Bong Kang, Jae-Kwan Hwang, Kwang Ho Lee

Research output: Contribution to journalArticle

Abstract

Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found to attenuate allergic responses in in vitro and in vivo experimental models. Ginseng has been reported to also possess various biological functions including anti-inflammatory activity. The present study was aimed at comparing the anti-allergic effect of ginseng and fermented ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line and in vivo in mice. Fermented ginseng extract (FPG) showed higher inhibitory effect against in vitro and in vivo allergic responses when compared with ginseng extract (PG). The secretion of β-hexosaminidase and interleukin (IL)-4 from the IgE-DNP-stimulated RBH-2H3 mast cells were significantly (p < 0.05) inhibited by FPG treatment, and this effect was concentration-dependent. Further, MKK4 activation and subsequent JNK phosphorylation were attenuated by FPG treatment. The inhibitory effect of FPG on the in vitro allergic response was verified in vivo against IgE-DNP–induced passive cutaneous anaphylaxis in a mouse model. These data indicated that the fermentation of ginseng with L. plantarum enhanced its anti-allergic effects both in vitro and in vivo. We predict that compositional changes in the ginsenosides caused by the fermentation may contribute to the change in the anti-allergic effects of ginseng. The results of our study highlight the potential of the use of FPG as a potential anti-allergic agent.

Original languageEnglish
Pages (from-to)1626-1634
Number of pages9
JournalJournal of microbiology and biotechnology
Volume28
Issue number10
DOIs
Publication statusPublished - 2018 Jan 1

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Anti-Allergic Agents
Passive Cutaneous Anaphylaxis
Panax
Fermentation
Immunoglobulin E
Lactobacillus plantarum
Ginsenosides
In Vitro Techniques
Hexosaminidases
Mast Cells
Interleukin-4
Anti-Inflammatory Agents
Theoretical Models
Phosphorylation
Cell Line

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Hwang, Seon Weon ; Sun, Xiao ; Han, Jun Hyuk ; Kim, Tae Yeon ; Koppula, Sushruta ; Kang, Tae Bong ; Hwang, Jae-Kwan ; Lee, Kwang Ho. / Fermentation-mediated enhancement of Ginseng’s anti-allergic activity against ige-mediated passive cutaneous anaphylaxis in vivo and in vitro. In: Journal of microbiology and biotechnology. 2018 ; Vol. 28, No. 10. pp. 1626-1634.
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Fermentation-mediated enhancement of Ginseng’s anti-allergic activity against ige-mediated passive cutaneous anaphylaxis in vivo and in vitro. / Hwang, Seon Weon; Sun, Xiao; Han, Jun Hyuk; Kim, Tae Yeon; Koppula, Sushruta; Kang, Tae Bong; Hwang, Jae-Kwan; Lee, Kwang Ho.

In: Journal of microbiology and biotechnology, Vol. 28, No. 10, 01.01.2018, p. 1626-1634.

Research output: Contribution to journalArticle

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