Fibrillarin binds to a 3′ cis-regulatory element in pre-mRNA of uvi15+ in fission yeast

Yeun Kyu Jang, Minkyu Kim, Sang Dai Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

uvi15+ is induced by various stresses including exposure to UV-light. Previously, we demonstrated that the UV-induction is mainly regulated at the post-transcriptional level through a cis-acting element in the pre-mRNA. Here we show that deletion analyses define an 18-nt element responsible for the UV-induction. RNA gel mobility shift assay showed that a specific protein(s) could form a complex with the 54-nt element but its binding ability is moderately decreased in response to UV-light. Using yeast three-hybrid screen, we isolated a homolog of fibrillarin as a protein interacting with the 54-nt element, which is a key nucleolar protein for pre-rRNA processing. We further showed that the recombinant fibrillarin specifically binds to the element in a sequence-specific manner. Thus, the data suggest that fission yeast fibrillarin might regulate uvi15+ mRNA stability via binding with the 54-nt element in the pre-mRNA, implying that fibrillarin is involved in both pre-mRNA and pre-rRNA processing.

Original languageEnglish
Pages (from-to)1184-1190
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number5
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Fibrillarin binds to a 3′ cis-regulatory element in pre-mRNA of uvi15<sup>+</sup> in fission yeast'. Together they form a unique fingerprint.

Cite this