Field-flow fractionation in bioanalysis: A review of recent trends

Barbara Roda, Andrea Zattoni, Pierluigi Reschiglian, Myeong Hee Moon, Mara Mirasoli, Elisa Michelini, Aldo Roda

Research output: Contribution to journalReview article

136 Citations (Scopus)

Abstract

Field-flow fractionation (FFF) is a mature technique in bioanalysis, and the number of applications to proteins and protein complexes, viruses, derivatized nano- and micronsized beads, sub-cellular units, and whole cell separation is constantly increasing. This can be ascribed to the non-invasivity of FFF when directly applied to biosamples. FFF is carried out in an open-channel structure by a flow stream of a mobile phase of any composition, and it is solely based on the interaction of the analytes with a perpendicularly applied field. For these reasons, fractionation is developed without surface interaction of the analyte with packing or gel media and without using degrading mobile phases. The fractionation device can be also easily sterilized, and analytes can be maintained under a bio-friendly environment. This allows to maintain native conditions of the sample in solution. In this review, FFF principles are briefly described, and some pioneering developments and applications in the bioanalytical field are tabled before detailed report of most recent FFF applications obtained also with the hyphenation of FFF with highly specific, sensitive characterization methods. Special focus is finally given to the emerging use of FFF as a pre-analytical step for mass-based identification and characterization of proteins and protein complexes in proteomics.

Original languageEnglish
Pages (from-to)132-143
Number of pages12
JournalAnalytica Chimica Acta
Volume635
Issue number2
DOIs
Publication statusPublished - 2009 Mar 9

Fingerprint

Field Flow Fractionation
Fractionation
flow field
Flow fields
fractionation
protein
Proteins
Cell Separation
trend
Proteomics
proteomics
Viruses
Gels
streamflow
virus
Equipment and Supplies
gel

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Roda, B., Zattoni, A., Reschiglian, P., Moon, M. H., Mirasoli, M., Michelini, E., & Roda, A. (2009). Field-flow fractionation in bioanalysis: A review of recent trends. Analytica Chimica Acta, 635(2), 132-143. https://doi.org/10.1016/j.aca.2009.01.015
Roda, Barbara ; Zattoni, Andrea ; Reschiglian, Pierluigi ; Moon, Myeong Hee ; Mirasoli, Mara ; Michelini, Elisa ; Roda, Aldo. / Field-flow fractionation in bioanalysis : A review of recent trends. In: Analytica Chimica Acta. 2009 ; Vol. 635, No. 2. pp. 132-143.
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Roda, B, Zattoni, A, Reschiglian, P, Moon, MH, Mirasoli, M, Michelini, E & Roda, A 2009, 'Field-flow fractionation in bioanalysis: A review of recent trends', Analytica Chimica Acta, vol. 635, no. 2, pp. 132-143. https://doi.org/10.1016/j.aca.2009.01.015

Field-flow fractionation in bioanalysis : A review of recent trends. / Roda, Barbara; Zattoni, Andrea; Reschiglian, Pierluigi; Moon, Myeong Hee; Mirasoli, Mara; Michelini, Elisa; Roda, Aldo.

In: Analytica Chimica Acta, Vol. 635, No. 2, 09.03.2009, p. 132-143.

Research output: Contribution to journalReview article

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AU - Roda, Barbara

AU - Zattoni, Andrea

AU - Reschiglian, Pierluigi

AU - Moon, Myeong Hee

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AU - Michelini, Elisa

AU - Roda, Aldo

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Roda B, Zattoni A, Reschiglian P, Moon MH, Mirasoli M, Michelini E et al. Field-flow fractionation in bioanalysis: A review of recent trends. Analytica Chimica Acta. 2009 Mar 9;635(2):132-143. https://doi.org/10.1016/j.aca.2009.01.015