Fine-tuning of epithelial taste bud organoid to promote functional recapitulation of taste reactivity

Anish Ashok Adpaikar, Sushan Zhang, Hyun Yi Kim, Ki Woo Kim, Seok Jun Moon, Jong Min Lee, Han Sung Jung

Research output: Contribution to journalArticlepeer-review

Abstract

Taste stem/progenitor cells from posterior mouse tongues have been used to generate taste bud organoids. However, the inaccessible location of taste receptor cells is observed in conventional organoids. In this study, we established a suspension-culture method to fine-tune taste bud organoids by apicobasal polarity alteration to form the accessible localization of taste receptor cells. Compared to conventional Matrigel-embedded organoids, suspension-cultured organoids showed comparable differentiation and renewal rates to those of taste buds in vivo and exhibited functional taste receptor cells and cycling progenitor cells. Accessible taste receptor cells enabled the direct application of calcium imaging to evaluate the taste response. Moreover, suspension-cultured organoids can be genetically altered. Suspension-cultured taste bud organoids harmoniously integrated with the recipient lingual epithelium, maintaining the taste receptor cells and gustatory innervation capacity. We propose that suspension-cultured organoids may provide an efficient model for taste research, including taste bud development, regeneration, and transplantation.

Original languageEnglish
Article number211
JournalCellular and Molecular Life Sciences
Volume79
Issue number4
DOIs
Publication statusPublished - 2022 Apr

Bibliographical note

Funding Information:
The National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MSIP) (NRF‐2016R1A5A2008630, NRF-2022R1A2B5B03001627 and NRF-2021R1A2C1005506) supported this work.

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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