First report of the carbapenemase gene blaOXA-499 in Acinetobacter pittii

Roshan D'Souza; Naina Adren Pinto; Paul G. Higgins; Insik Hwang; Dongeun Yong; Jongrak Choi; Kyoungwon Lee; Yunsop Chong

doi:10.1128/AAC.02676-16

First report of the carbapenemase gene bla_OXA-499 in Acinetobacter pittii

Roshan D'Souza, Naina Adren Pinto, Paul G. Higgins, Insik Hwang, Dongeun Yong, Jongrak Choi, Kyoungwon Lee, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We identified the carbapenemase gene bla_OXA-499, a variant of bla_OXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.

Original language	English
Article number	e02676
Journal	Antimicrobial agents and chemotherapy
Volume	61
Issue number	5
DOIs	https://doi.org/10.1128/AAC.02676-16
Publication status	Published - 2017 May

Bibliographical note

Funding Information:
This work was supported by the BioNano Health-Guard Research Center, funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korea as a Global Frontier Project (grant H-GUARD-2014M3A6B2060509); by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University; and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (grant 2014M3A9E5073818).

Publisher Copyright:
Copyright © 2017 American Society for Microbiology. All Rights Reserved.

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/AAC.02676-16

Cite this

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title = "First report of the carbapenemase gene blaOXA-499 in Acinetobacter pittii",

abstract = "We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.",

author = "Roshan D'Souza and Pinto, {Naina Adren} and Higgins, {Paul G.} and Insik Hwang and Dongeun Yong and Jongrak Choi and Kyoungwon Lee and Yunsop Chong",

note = "Funding Information: This work was supported by the BioNano Health-Guard Research Center, funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korea as a Global Frontier Project (grant H-GUARD-2014M3A6B2060509); by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University; and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (grant 2014M3A9E5073818). Publisher Copyright: Copyright {\textcopyright} 2017 American Society for Microbiology. All Rights Reserved.",

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AU - Pinto, Naina Adren

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AU - Hwang, Insik

AU - Yong, Dongeun

AU - Choi, Jongrak

AU - Lee, Kyoungwon

AU - Chong, Yunsop

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AB - We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.

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