First report of the carbapenemase gene blaOXA-499 in Acinetobacter pittii

Roshan D'Souza; Naina Adren Pinto; Paul G. Higgins; Insik Hwang; Dongeun Yong; Jongrak Choi; Kyoungwon Lee; Yunsop Chong

doi:10.1128/AAC.02676-16

First report of the carbapenemase gene bla_OXA-499 in Acinetobacter pittii

Roshan D'Souza, Naina Adren Pinto, Paul G. Higgins, Insik Hwang, Dongeun Yong, Jongrak Choi, Kyoungwon Lee, Yunsop Chong

Department of Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We identified the carbapenemase gene bla_OXA-499, a variant of bla_OXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.

Original language	English
Article number	e02676
Journal	Antimicrobial agents and chemotherapy
Volume	61
Issue number	5
DOIs	https://doi.org/10.1128/AAC.02676-16
Publication status	Published - 2017 May

Bibliographical note

Funding Information:
This work was supported by the BioNano Health-Guard Research Center, funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korea as a Global Frontier Project (grant H-GUARD-2014M3A6B2060509); by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University; and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (grant 2014M3A9E5073818).

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmacology (medical)
Infectious Diseases

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title = "First report of the carbapenemase gene blaOXA-499 in Acinetobacter pittii",

abstract = "We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.",

author = "Roshan D'Souza and Pinto, {Naina Adren} and Higgins, {Paul G.} and Insik Hwang and Dongeun Yong and Jongrak Choi and Kyoungwon Lee and Yunsop Chong",

note = "Funding Information: This work was supported by the BioNano Health-Guard Research Center, funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korea as a Global Frontier Project (grant H-GUARD-2014M3A6B2060509); by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University; and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (grant 2014M3A9E5073818).",

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AU - D'Souza, Roshan

AU - Pinto, Naina Adren

AU - Higgins, Paul G.

AU - Hwang, Insik

AU - Yong, Dongeun

AU - Choi, Jongrak

AU - Lee, Kyoungwon

AU - Chong, Yunsop

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AB - We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.

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