We identified the carbapenemase gene blaOXA-499, a variant of blaOXA-143, from a clinical isolate of Acinetobacter pittii for the first time. OXA-499 shared 93.1% amino acid identity with OXA-143, and the gene was located on the chromosome. By cloning the OXA-499-encoding gene into the pWH1266 vector and transforming it into susceptible Acinetobacter spp., we were able to show that OXA-499 confers resistance to carbapenems.
Bibliographical noteFunding Information:
This work was supported by the BioNano Health-Guard Research Center, funded by the Ministry of Science, ICT and Future Planning (MSIP) of Korea as a Global Frontier Project (grant H-GUARD-2014M3A6B2060509); by a grant from the Brain Korea 21 PLUS Project for Medical Science, Yonsei University; and by the Bio & Medical Technology Development Program of the NRF funded by the Korean government (grant 2014M3A9E5073818).
All Science Journal Classification (ASJC) codes
- Pharmacology (medical)
- Infectious Diseases