Fission yeast Rap1 homolog is a telomere-specific silencing factor and interacts with Taz1p

Min Jung Park, Yeun Kyu Jang, Eun Shik Choi, Hyun Soo Kim, Sang Dai Park

Research output: Contribution to journalArticle

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Abstract

Taz1p is the Fission yeast orthologue of human TRF2, a telomeric repeat-binding protein. Δtaz1 mutants are defective in telomeric silencing, telomere length control, and meiotic recombination events. A recent report demonstrated that the human Rap1p homolog (hRap1) is recruited to telomere by interaction with TRF2, arguing that the telomere control mechanism of higher eukaryotes is distinct from that of the budding yeast. Taz1p showed a significant similarity to human TRF2, but not with the budding yeast Rap1p (scRap1p). This suggests that Taz1p and TRF2 share common features in telomere regulation. To assess the roles of Taz1p in telomere-related functions in detail, we attempted to identify a protein(s) that interacts with Taz1p by using two-hybrid screening. Interestingly, the sequence analysis of a positive clone revealed a perfect match with a Rap1 homolog in S. pombe (spRap1), which showed a significant homology with scRap1p and hRap1p. Here we show that the spRap1 deficiency in haploid cells is viable, which results in increased telomere length regulation, disruption of telomere silencing, and aberrant meiosis (like the Δtaz1 mutant). This suggests that spRap1p might be recruited to the telomere by Taz1p and play crucial roles in telomere function. Interestingly, the Δrap1 mutants in fission yeast are defective only for telomere silencing. Therefore, the role of spRap1p may be distinct from that of scRap1p, which is involved in the silencing at both the telomere and mating type locus. Our data, therefore, suggest that the regulation mechanisms of telomere in fission yeast resemble that of higher eukaryotic cells rather than the budding yeast.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalMolecules and Cells
Volume13
Issue number2
Publication statusPublished - 2002 Apr 1

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Schizosaccharomyces
Telomere
Saccharomycetales
Haploidy
Meiosis
Eukaryotic Cells
Eukaryota
Genetic Recombination
Sequence Analysis
Carrier Proteins
Clone Cells

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Park, Min Jung ; Jang, Yeun Kyu ; Choi, Eun Shik ; Kim, Hyun Soo ; Park, Sang Dai. / Fission yeast Rap1 homolog is a telomere-specific silencing factor and interacts with Taz1p. In: Molecules and Cells. 2002 ; Vol. 13, No. 2. pp. 327-333.
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abstract = "Taz1p is the Fission yeast orthologue of human TRF2, a telomeric repeat-binding protein. Δtaz1 mutants are defective in telomeric silencing, telomere length control, and meiotic recombination events. A recent report demonstrated that the human Rap1p homolog (hRap1) is recruited to telomere by interaction with TRF2, arguing that the telomere control mechanism of higher eukaryotes is distinct from that of the budding yeast. Taz1p showed a significant similarity to human TRF2, but not with the budding yeast Rap1p (scRap1p). This suggests that Taz1p and TRF2 share common features in telomere regulation. To assess the roles of Taz1p in telomere-related functions in detail, we attempted to identify a protein(s) that interacts with Taz1p by using two-hybrid screening. Interestingly, the sequence analysis of a positive clone revealed a perfect match with a Rap1 homolog in S. pombe (spRap1), which showed a significant homology with scRap1p and hRap1p. Here we show that the spRap1 deficiency in haploid cells is viable, which results in increased telomere length regulation, disruption of telomere silencing, and aberrant meiosis (like the Δtaz1 mutant). This suggests that spRap1p might be recruited to the telomere by Taz1p and play crucial roles in telomere function. Interestingly, the Δrap1 mutants in fission yeast are defective only for telomere silencing. Therefore, the role of spRap1p may be distinct from that of scRap1p, which is involved in the silencing at both the telomere and mating type locus. Our data, therefore, suggest that the regulation mechanisms of telomere in fission yeast resemble that of higher eukaryotic cells rather than the budding yeast.",
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Fission yeast Rap1 homolog is a telomere-specific silencing factor and interacts with Taz1p. / Park, Min Jung; Jang, Yeun Kyu; Choi, Eun Shik; Kim, Hyun Soo; Park, Sang Dai.

In: Molecules and Cells, Vol. 13, No. 2, 01.04.2002, p. 327-333.

Research output: Contribution to journalArticle

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AU - Jang, Yeun Kyu

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N2 - Taz1p is the Fission yeast orthologue of human TRF2, a telomeric repeat-binding protein. Δtaz1 mutants are defective in telomeric silencing, telomere length control, and meiotic recombination events. A recent report demonstrated that the human Rap1p homolog (hRap1) is recruited to telomere by interaction with TRF2, arguing that the telomere control mechanism of higher eukaryotes is distinct from that of the budding yeast. Taz1p showed a significant similarity to human TRF2, but not with the budding yeast Rap1p (scRap1p). This suggests that Taz1p and TRF2 share common features in telomere regulation. To assess the roles of Taz1p in telomere-related functions in detail, we attempted to identify a protein(s) that interacts with Taz1p by using two-hybrid screening. Interestingly, the sequence analysis of a positive clone revealed a perfect match with a Rap1 homolog in S. pombe (spRap1), which showed a significant homology with scRap1p and hRap1p. Here we show that the spRap1 deficiency in haploid cells is viable, which results in increased telomere length regulation, disruption of telomere silencing, and aberrant meiosis (like the Δtaz1 mutant). This suggests that spRap1p might be recruited to the telomere by Taz1p and play crucial roles in telomere function. Interestingly, the Δrap1 mutants in fission yeast are defective only for telomere silencing. Therefore, the role of spRap1p may be distinct from that of scRap1p, which is involved in the silencing at both the telomere and mating type locus. Our data, therefore, suggest that the regulation mechanisms of telomere in fission yeast resemble that of higher eukaryotic cells rather than the budding yeast.

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