We have synthesized a flavin-N(5)-oxide derivative with a p-toluenesulfonyl (Ts-OF) group as a “turn-on” fluorescent probe for the detection of several antioxidant amino acids and biothiols. Oxidized flavin was synthesized by using dithiothreitol as the reducing agent. Ts-OF showed a light-driven fluorescence enhancement in the presence of several amino acids and biothiols such as histidine (His), methionine (Met), cysteine (Cys), glutathione (GSH), and homocysteine (Hcy). The 1H NMR study indicated the reductive elimination of the p-toluenesulfonyl group from Ts-OF in the presence of antioxidants and photo-irradiation.
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This work was supported by the Mid-Career Researcher Program (2014R1A2A1A10051083) funded by the National Research Foundation (NRF) of Korea, Ministry of Science, ICT & Future, Korea.
© 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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