Flavobacterium dauae sp. Nov., isolated from rhizosphere soil of a tomato plant

Eun Joo Jung, Soo Yeon Choi, Sang Moo Lee, Ju Yeon Song, Hyoung Ju Lee, Eunjin Kim, Pyeong An Lee, Kihyuck Choi, Jihyun F. Kim, Seon Woo Lee

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A bacterial strain, designated TCH3-2T, was isolated from the rhizosphere of tomato plant grown at Dong-A University Agri-cultural Experiment Station, Republic of Korea. The strain was Gram-stain-negative, obligate aerobic, orange yellow-coloured, motile by gliding and short rod-shaped. Strain TCH3-2T only grew on 1/2 tryptic soy agar and Luria–Bertani agar among the media tested, with optimum growth at 28 °C and pH 7. Salt of 1% NaCl was necessary to support the growth of TCH3-2T. Strain TCH3-2T produced flexirubin-type pigments. The predominant cellular fatty acids were iso-C15:0 (55.6%), iso-C17:0 3-OH (17.9%), summed feature 9 (comprising C16:0 10-methyl and/or iso-C17:1 ω9c; 10.5%), iso-C15:0 3-OH (4.8%) and anteiso-C15:0 (2.3%). The major menaquinone was menaquinone-6 and the major polar lipids were phosphatidylethanolamine, five unknown aminolip-ids and three unknown lipids. Phylogenetic analysis based on 16S rRNA sequences indicated that TCH3-2T was closely related to Flavobacterium ummariense DS-12T (95.16%), Flavobacterium marinum SW105T (95.14%) and Flavobacterium viscosus YIM 102796T (94.54%). The draft genome of TCH3-2T comprised ca. 2.8 Mb with a G+C content of 34.61mol%. The average nucleotide identity and digital DNA–DNA hybridization values between TCH3-2T and closely related Flavobacterium species showed that it belongs to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of TCH3-2T from its closest relatives. Thus, chemotaxonomic characteristics together with phylogenetic affiliation illustrate that TCH3-2T represents a novel species of the genus Flavobacterium, for which the name Flavobacte-rium dauae sp. nov. (type strain TCH3-2T=KACC 19054T=JCM 34025T) is proposed.

Original languageEnglish
Article number005033
JournalInternational Journal of Systematic and Evolutionary Microbiology
Issue number10
Publication statusPublished - 2021

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program to S.-W. L. (PJ01313101) through the Rural Development Administration, and by National Research Foundation of Korea (NRF) grants to S.-W. L. (No. 2020R1A2C3005453 and 2020R1A6A1A03047729) funded by the Korea government (MSIT and MOE), Republic of Korea.

Publisher Copyright:
© 2021 The Authors.

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics


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