Flexible microelectrode array for interfacing with the surface of neural ganglia

Zachariah J. Sperry, Kyounghwan Na, Saman S. Parizi, Hillel J. Chiel, John Seymour, Euisik Yoon, Tim M. Bruns

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective. The dorsal root ganglia (DRG) are promising nerve structures for sensory neural interfaces because they provide centralized access to primary afferent cell bodies and spinal reflex circuitry. In order to harness this potential, new electrode technologies are needed which take advantage of the unique properties of DRG, specifically the high density of neural cell bodies at the dorsal surface. Here we report initial in vivo results from the development of a flexible non-penetrating polyimide electrode array interfacing with the surface of ganglia. Approach. Multiple layouts of a 64-channel iridium electrode (420 m2) array were tested, with pitch as small as 25 m. The buccal ganglia of invertebrate sea slug Aplysia californica were used to develop handling and recording techniques with ganglionic surface electrode arrays (GSEAs). We also demonstrated the GSEA's capability to record single- and multi-unit activity from feline lumbosacral DRG related to a variety of sensory inputs, including cutaneous brushing, joint flexion, and bladder pressure. Main results. We recorded action potentials from a variety of Aplysia neurons activated by nerve stimulation, and units were observed firing simultaneously on closely spaced electrode sites. We also recorded single- and multi-unit activity associated with sensory inputs from feline DRG. We utilized spatial oversampling of action potentials on closely-spaced electrode sites to estimate the location of neural sources at between 25 m and 107 m below the DRG surface. We also used the high spatial sampling to demonstrate a possible spatial sensory map of one feline's DRG. We obtained activation of sensory fibers with low-amplitude stimulation through individual or groups of GSEA electrode sites. Significance. Overall, the GSEA has been shown to provide a variety of information types from ganglia neurons and to have significant potential as a tool for neural mapping and interfacing.

Original languageEnglish
Article number036027
JournalJournal of Neural Engineering
Volume15
Issue number3
DOIs
Publication statusPublished - 2018 Apr 16

Fingerprint

Microelectrodes
Ganglia
Electrodes
Spinal Ganglia
Felidae
Aplysia
Invertebrate Ganglia
Action Potentials
Neurons
Cells
Iridium
Gastropoda
Cheek
Polyimides
Oceans and Seas
Reflex
Urinary Bladder
Joints
Chemical activation
Sampling

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Sperry, Z. J., Na, K., Parizi, S. S., Chiel, H. J., Seymour, J., Yoon, E., & Bruns, T. M. (2018). Flexible microelectrode array for interfacing with the surface of neural ganglia. Journal of Neural Engineering, 15(3), [036027]. https://doi.org/10.1088/1741-2552/aab55f
Sperry, Zachariah J. ; Na, Kyounghwan ; Parizi, Saman S. ; Chiel, Hillel J. ; Seymour, John ; Yoon, Euisik ; Bruns, Tim M. / Flexible microelectrode array for interfacing with the surface of neural ganglia. In: Journal of Neural Engineering. 2018 ; Vol. 15, No. 3.
@article{05bb7d734d3d4217a21c8764db2d744c,
title = "Flexible microelectrode array for interfacing with the surface of neural ganglia",
abstract = "Objective. The dorsal root ganglia (DRG) are promising nerve structures for sensory neural interfaces because they provide centralized access to primary afferent cell bodies and spinal reflex circuitry. In order to harness this potential, new electrode technologies are needed which take advantage of the unique properties of DRG, specifically the high density of neural cell bodies at the dorsal surface. Here we report initial in vivo results from the development of a flexible non-penetrating polyimide electrode array interfacing with the surface of ganglia. Approach. Multiple layouts of a 64-channel iridium electrode (420 m2) array were tested, with pitch as small as 25 m. The buccal ganglia of invertebrate sea slug Aplysia californica were used to develop handling and recording techniques with ganglionic surface electrode arrays (GSEAs). We also demonstrated the GSEA's capability to record single- and multi-unit activity from feline lumbosacral DRG related to a variety of sensory inputs, including cutaneous brushing, joint flexion, and bladder pressure. Main results. We recorded action potentials from a variety of Aplysia neurons activated by nerve stimulation, and units were observed firing simultaneously on closely spaced electrode sites. We also recorded single- and multi-unit activity associated with sensory inputs from feline DRG. We utilized spatial oversampling of action potentials on closely-spaced electrode sites to estimate the location of neural sources at between 25 m and 107 m below the DRG surface. We also used the high spatial sampling to demonstrate a possible spatial sensory map of one feline's DRG. We obtained activation of sensory fibers with low-amplitude stimulation through individual or groups of GSEA electrode sites. Significance. Overall, the GSEA has been shown to provide a variety of information types from ganglia neurons and to have significant potential as a tool for neural mapping and interfacing.",
author = "Sperry, {Zachariah J.} and Kyounghwan Na and Parizi, {Saman S.} and Chiel, {Hillel J.} and John Seymour and Euisik Yoon and Bruns, {Tim M.}",
year = "2018",
month = "4",
day = "16",
doi = "10.1088/1741-2552/aab55f",
language = "English",
volume = "15",
journal = "Journal of Neural Engineering",
issn = "1741-2560",
publisher = "IOP Publishing Ltd.",
number = "3",

}

Flexible microelectrode array for interfacing with the surface of neural ganglia. / Sperry, Zachariah J.; Na, Kyounghwan; Parizi, Saman S.; Chiel, Hillel J.; Seymour, John; Yoon, Euisik; Bruns, Tim M.

In: Journal of Neural Engineering, Vol. 15, No. 3, 036027, 16.04.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Flexible microelectrode array for interfacing with the surface of neural ganglia

AU - Sperry, Zachariah J.

AU - Na, Kyounghwan

AU - Parizi, Saman S.

AU - Chiel, Hillel J.

AU - Seymour, John

AU - Yoon, Euisik

AU - Bruns, Tim M.

PY - 2018/4/16

Y1 - 2018/4/16

N2 - Objective. The dorsal root ganglia (DRG) are promising nerve structures for sensory neural interfaces because they provide centralized access to primary afferent cell bodies and spinal reflex circuitry. In order to harness this potential, new electrode technologies are needed which take advantage of the unique properties of DRG, specifically the high density of neural cell bodies at the dorsal surface. Here we report initial in vivo results from the development of a flexible non-penetrating polyimide electrode array interfacing with the surface of ganglia. Approach. Multiple layouts of a 64-channel iridium electrode (420 m2) array were tested, with pitch as small as 25 m. The buccal ganglia of invertebrate sea slug Aplysia californica were used to develop handling and recording techniques with ganglionic surface electrode arrays (GSEAs). We also demonstrated the GSEA's capability to record single- and multi-unit activity from feline lumbosacral DRG related to a variety of sensory inputs, including cutaneous brushing, joint flexion, and bladder pressure. Main results. We recorded action potentials from a variety of Aplysia neurons activated by nerve stimulation, and units were observed firing simultaneously on closely spaced electrode sites. We also recorded single- and multi-unit activity associated with sensory inputs from feline DRG. We utilized spatial oversampling of action potentials on closely-spaced electrode sites to estimate the location of neural sources at between 25 m and 107 m below the DRG surface. We also used the high spatial sampling to demonstrate a possible spatial sensory map of one feline's DRG. We obtained activation of sensory fibers with low-amplitude stimulation through individual or groups of GSEA electrode sites. Significance. Overall, the GSEA has been shown to provide a variety of information types from ganglia neurons and to have significant potential as a tool for neural mapping and interfacing.

AB - Objective. The dorsal root ganglia (DRG) are promising nerve structures for sensory neural interfaces because they provide centralized access to primary afferent cell bodies and spinal reflex circuitry. In order to harness this potential, new electrode technologies are needed which take advantage of the unique properties of DRG, specifically the high density of neural cell bodies at the dorsal surface. Here we report initial in vivo results from the development of a flexible non-penetrating polyimide electrode array interfacing with the surface of ganglia. Approach. Multiple layouts of a 64-channel iridium electrode (420 m2) array were tested, with pitch as small as 25 m. The buccal ganglia of invertebrate sea slug Aplysia californica were used to develop handling and recording techniques with ganglionic surface electrode arrays (GSEAs). We also demonstrated the GSEA's capability to record single- and multi-unit activity from feline lumbosacral DRG related to a variety of sensory inputs, including cutaneous brushing, joint flexion, and bladder pressure. Main results. We recorded action potentials from a variety of Aplysia neurons activated by nerve stimulation, and units were observed firing simultaneously on closely spaced electrode sites. We also recorded single- and multi-unit activity associated with sensory inputs from feline DRG. We utilized spatial oversampling of action potentials on closely-spaced electrode sites to estimate the location of neural sources at between 25 m and 107 m below the DRG surface. We also used the high spatial sampling to demonstrate a possible spatial sensory map of one feline's DRG. We obtained activation of sensory fibers with low-amplitude stimulation through individual or groups of GSEA electrode sites. Significance. Overall, the GSEA has been shown to provide a variety of information types from ganglia neurons and to have significant potential as a tool for neural mapping and interfacing.

UR - http://www.scopus.com/inward/record.url?scp=85047779060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047779060&partnerID=8YFLogxK

U2 - 10.1088/1741-2552/aab55f

DO - 10.1088/1741-2552/aab55f

M3 - Article

C2 - 29521279

AN - SCOPUS:85047779060

VL - 15

JO - Journal of Neural Engineering

JF - Journal of Neural Engineering

SN - 1741-2560

IS - 3

M1 - 036027

ER -