Flexible nanocellulose-based SERS substrates for fast analysis of hazardous materials by spiral scanning

Surface-enhanced Raman scattering (SERS) has proven to be a valuable tool for assessing harmful chemicals in various substances, including water, soil, and foods. However, a fast measurement system is required for multiplexed detection to extend the range of its applications. The rotating scanning stage of the SERS substrate is considered to be a promising approach to achieving a fast measurement system. This paper reports a facile measurement system by using a flexible nanocellulose-based SERS substrate and a spiral scanning system, which rotates the cylinder sample holder and moves the stage. A flexible nanocellulose-based SERS substrate deposited with Au nanoparticles is suitable for the spiral scanning system, which requires SERS substrates to be highly flexible and durable. The well-known toxic fungicide, thiram, was tested by this system. The results revealed that the nanocellulose-based SERS substrate is well-fitted with a spiral scanning system and that the signal data from a large area substrate can be obtained within 30 s. It is noteworthy that the error of spiral scanning measurements is smaller than that of multi-spot sampling. This work provides a powerful tool for Raman spectroscopic analysis, which requires quantitative and fast testing. Furthermore, various flexible SERS substrates can be utilized in this system for toxic materials detection.