Flow cytometric immunoassay using E. coli with autodisplayed Z-domains

Min Park, Ji Hong Bong, Gu Yoo, Joachim Jose, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Recently, we reported a highly sensitive immunoassay using Escherichia coli cells with autodisplayed Z-domains. In this work, E. coli cells with autodisplayed Z-domains were applied to the flow-cytometry-based simultaneous detection of multiple analytes. The E. coli cells were doubly transfected to express a fluorescent protein (tdTomato) in the cytosol and the autodisplayed Z-domains on the outer membrane. By using E. coli cells with only the autodisplayed Z-domains, immunoassay of multiple analytes could be performed simultaneously on the same sample. Flow cytometry can be used to identify the immunoassay type by simultaneously detecting the fluorescence signal from the cytosol (tdTomato) and the fluorescence from the outer membrane, enabling the quantification of bound analytes after treatment with additional fluorescently labeled antibodies. To demonstrate the immunoassay of multiple analytes by using flow cytometry, human hepatitis B virus surface antigen (HBsAg) and C-reactive protein (CRP), a broad spectrum inflammation marker, were used as model analytes.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalEnzyme and Microbial Technology
Volume53
Issue number3
DOIs
Publication statusPublished - 2013 Aug 15

Fingerprint

Immunoassay
Escherichia coli
Flow cytometry
Flow Cytometry
Cytosol
Fluorescence
Proteins
Membranes
Surface Antigens
Antigens
Hepatitis B Surface Antigens
Viruses
Hepatitis B virus
Antibodies
C-Reactive Protein
Inflammation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Park, Min ; Bong, Ji Hong ; Yoo, Gu ; Jose, Joachim ; Kang, Min Jung ; Pyun, Jae Chul. / Flow cytometric immunoassay using E. coli with autodisplayed Z-domains. In: Enzyme and Microbial Technology. 2013 ; Vol. 53, No. 3. pp. 181-188.
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Flow cytometric immunoassay using E. coli with autodisplayed Z-domains. / Park, Min; Bong, Ji Hong; Yoo, Gu; Jose, Joachim; Kang, Min Jung; Pyun, Jae Chul.

In: Enzyme and Microbial Technology, Vol. 53, No. 3, 15.08.2013, p. 181-188.

Research output: Contribution to journalArticle

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