Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Kyeonghye Guk; Seul Gee Hwang; Jaewoo Lim; Hye young Son; Yuna Choi; Yong Min Huh; Taejoon Kang; Juyeon Jung; Eun Kyung Lim

doi:10.1039/C9CC00351G

Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Kyeonghye Guk, Seul Gee Hwang, Jaewoo Lim, Hye young Son, Yuna Choi, Yong Min Huh, Taejoon Kang, Juyeon Jung, Eun Kyung Lim

Department of Radiology

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.

Original language	English
Pages (from-to)	3457-3460
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	24
DOIs	https://doi.org/10.1039/C9CC00351G
Publication status	Published - 2019

Bibliographical note

Funding Information:
This work was supported by the Center for BioNano HealthGuard funded by the Ministry of Science and ICT (MSIT) as Global Frontier Project (H-GUARD_2014M3A6B2060507 and H-GUARD_2013M3A6B2078950), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of the NRF funded by MSIT (NRF-2018R1C1B6005424), and KRIBB Research Initiative Program.

Publisher Copyright:
© The Royal Society of Chemistry.

All Science Journal Classification (ASJC) codes

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/C9CC00351G

Cite this

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title = "Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit",

abstract = "We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.",

author = "Kyeonghye Guk and Hwang, {Seul Gee} and Jaewoo Lim and Son, {Hye young} and Yuna Choi and Huh, {Yong Min} and Taejoon Kang and Juyeon Jung and Lim, {Eun Kyung}",

note = "Funding Information: This work was supported by the Center for BioNano HealthGuard funded by the Ministry of Science and ICT (MSIT) as Global Frontier Project (H-GUARD_2014M3A6B2060507 and H-GUARD_2013M3A6B2078950), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of the NRF funded by MSIT (NRF-2018R1C1B6005424), and KRIBB Research Initiative Program. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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language = "English",

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AU - Guk, Kyeonghye

AU - Hwang, Seul Gee

AU - Lim, Jaewoo

AU - Son, Hye young

AU - Choi, Yuna

AU - Huh, Yong Min

AU - Kang, Taejoon

AU - Jung, Juyeon

AU - Lim, Eun Kyung

N1 - Funding Information: This work was supported by the Center for BioNano HealthGuard funded by the Ministry of Science and ICT (MSIT) as Global Frontier Project (H-GUARD_2014M3A6B2060507 and H-GUARD_2013M3A6B2078950), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by MSIT (NRF-2018M3A9E2022821), the Basic Science Research Program of the NRF funded by MSIT (NRF-2018R1C1B6005424), and KRIBB Research Initiative Program. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.

AB - We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.

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Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit

Abstract

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