Abstract
We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.
| Original language | English |
|---|---|
| Pages (from-to) | 3457-3460 |
| Number of pages | 4 |
| Journal | Chemical Communications |
| Volume | 55 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 2019 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Catalysis
- Electronic, Optical and Magnetic Materials
- Ceramics and Composites
- Chemistry(all)
- Surfaces, Coatings and Films
- Metals and Alloys
- Materials Chemistry
Cite this
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Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit. / Guk, Kyeonghye; Hwang, Seul Gee; Lim, Jaewoo; Son, Hye young; Choi, Yuna; Huh, Yong Min; Kang, Taejoon; Jung, Juyeon; Lim, Eun Kyung.
In: Chemical Communications, Vol. 55, No. 24, 01.01.2019, p. 3457-3460.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fluorescence amplified sensing platforms enabling miRNA detection by self-circulation of a molecular beacon circuit
AU - Guk, Kyeonghye
AU - Hwang, Seul Gee
AU - Lim, Jaewoo
AU - Son, Hye young
AU - Choi, Yuna
AU - Huh, Yong Min
AU - Kang, Taejoon
AU - Jung, Juyeon
AU - Lim, Eun Kyung
PY - 2019/1/1
Y1 - 2019/1/1
N2 - We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.
AB - We have proposed a novel strategy for miRNA detection through enzyme-free signal amplification by self-circulation of the hybridization between the miRNAs and molecular beacon (MB) circuits. Unlike general MB-based miRNA detection based on the one-to-one (1 : 1) hybridization between MBs and miRNA, our system consists of four species of MBs (MBs A, B, C and D) (MB circuits) and is activated by a hybridization chain reaction. MBs stably coexist as hairpin structures that hardly show fluorescence signals in the absence of target miRNA. After miRNA detection, this MB circuit is able to generate fluorescence signals and amplify the fluorescence signal, contributing to improvement in detection sensitivity under iso-thermal conditions without an enzyme. Furthermore, in vitro and in vivo studies have proven that MB circuits can detect low levels of miRNA with high sensitivity, compared to when only one MB alone is used. Therefore, the MB circuits can provide a useful platform for target miRNA detection.
UR - http://www.scopus.com/inward/record.url?scp=85063096454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063096454&partnerID=8YFLogxK
U2 - 10.1039/C9CC00351G
DO - 10.1039/C9CC00351G
M3 - Article
C2 - 30735212
AN - SCOPUS:85063096454
VL - 55
SP - 3457
EP - 3460
JO - Chemical Communications
JF - Chemical Communications
SN - 1359-7345
IS - 24
ER -