Fluorescence-based detection of point mutation in DNA sequences by CdS quantum dot aggregation

Taehoon Kim, Minho Noh, Hosub Lee, Sang Woo Joo, So Yeong Lee, Kangtaek Lee

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We present a novel method for the detection of single base mismatch based on fluorescence quenching that unmodified CdS quantum dots exhibit upon aggregation. Target DNA sequences of interest are breast cancer 2 (BRCA2) and signal-induced proliferation-associated gene 1 (Sipal) sequences. We monitor aggregation of CdS quantum dots upon addition of double-stranded DNAs at different salt concentration using quasielastic light scattering (QELS), transmission electron microscopy (TEM), photoluminescence spectroscopy, and zeta potential measurement. Our results indicate that the double-stranded DNA with a perfectly matched sequence can easily be discerned by naked eye from the single base mismatched one due to the fluorescence quenching phenomenon caused by selective aggregation of the CdS quantum dots.

Original languageEnglish
Pages (from-to)14487-14490
Number of pages4
JournalJournal of Physical Chemistry B
Volume113
Issue number43
DOIs
Publication statusPublished - 2009 Oct 29

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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