TY - GEN
T1 - Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy
AU - De Luca, Anna C.
AU - Mazilu, Michael
AU - Riches, Andrew
AU - Herrington, Simon
AU - Dholakia, Kishan
PY - 2010
Y1 - 2010
N2 - The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.
AB - The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.
UR - http://www.scopus.com/inward/record.url?scp=77951715601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951715601&partnerID=8YFLogxK
U2 - 10.1117/12.841686
DO - 10.1117/12.841686
M3 - Conference contribution
AN - SCOPUS:77951715601
SN - 9780819479518
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Advanced Biomedical and Clinical Diagnostic Systems VIII
T2 - Advanced Biomedical and Clinical Diagnostic Systems VIII
Y2 - 24 January 2010 through 26 January 2010
ER -