Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Anna C. De Luca, Michael Mazilu, Andrew Riches, Simon Herrington, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
DOIs
Publication statusPublished - 2010 May 7
EventAdvanced Biomedical and Clinical Diagnostic Systems VIII - San Francisco, CA, United States
Duration: 2010 Jan 242010 Jan 26

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7555
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic Systems VIII
CountryUnited States
CitySan Francisco, CA
Period10/1/2410/1/26

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy'. Together they form a unique fingerprint.

  • Cite this

    De Luca, A. C., Mazilu, M., Riches, A., Herrington, S., & Dholakia, K. (2010). Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy. In Advanced Biomedical and Clinical Diagnostic Systems VIII [755517] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7555). https://doi.org/10.1117/12.841686