Fluorescence-guided surgery with splenic preservation prevents tumor recurrence in an orthotopic nude-mouse model of human pancreatic cancer

Ho Kyoung Hwang, ChangMoo Kang, Sung Hwan Lee, Takashi Murakami, Tasuku Kiyuna, SeHoon Kim, Robert M. Hoffman, Michael Bouvet

Research output: Contribution to journalArticle

Abstract

Aim: The purpose of this study was to investigate whether splenectomy influences tumor recurrence after fluorescence-guided surgery (FGS) in an orthotopic nude-mouse model of pancreatic cancer. Materials and Methods: Green fluorescence protein (GFP)-labeled human pancreatic cancer cells (MiaPaCa2-GFP) were subcutaneously injected into the flanks of nude mice. Subcutaneous tumors were harvested and surgical orthotopic implantation (SOI) was performed in the tail of the pancreas with small tumor fragments. FGS was performed 21 days after SOI. Mice were then randomly divided into FGS-only control group (n=7) and FGS plus splenectomy group (n=8). Tumor recurrence was analyzed by laparotomy 21 days after FGS. Results: In the control group, no recurrence was found. In contrast, multiple peritoneal seeded nodules were observed in two mice of the splenectomy group (0% vs. 25%, p=0.467). Conclusion: Postoperative tumor recurrence only occurred in the splenectomy-treated group suggesting that FGS can spare the patient the morbidity of splenectomy.

Original languageEnglish
Pages (from-to)665-670
Number of pages6
JournalAnticancer research
Volume38
Issue number2
DOIs
Publication statusPublished - 2018 Feb 1

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Pancreatic Neoplasms
Nude Mice
Fluorescence
Splenectomy
Recurrence
Neoplasms
Control Groups
Laparotomy
Tail
Pancreas
Proteins
Morbidity

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Hwang, Ho Kyoung ; Kang, ChangMoo ; Lee, Sung Hwan ; Murakami, Takashi ; Kiyuna, Tasuku ; Kim, SeHoon ; Hoffman, Robert M. ; Bouvet, Michael. / Fluorescence-guided surgery with splenic preservation prevents tumor recurrence in an orthotopic nude-mouse model of human pancreatic cancer. In: Anticancer research. 2018 ; Vol. 38, No. 2. pp. 665-670.
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Fluorescence-guided surgery with splenic preservation prevents tumor recurrence in an orthotopic nude-mouse model of human pancreatic cancer. / Hwang, Ho Kyoung; Kang, ChangMoo; Lee, Sung Hwan; Murakami, Takashi; Kiyuna, Tasuku; Kim, SeHoon; Hoffman, Robert M.; Bouvet, Michael.

In: Anticancer research, Vol. 38, No. 2, 01.02.2018, p. 665-670.

Research output: Contribution to journalArticle

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AU - Kim, SeHoon

AU - Hoffman, Robert M.

AU - Bouvet, Michael

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