Fluorescence lifetime determination of photo-bleaching correlation

Won Sang Hwang, Young Sik Song, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

FLIM is relatively stable about photo-bleaching effect. In case of dye lias multi-exponential decay property, fluorescence lifetime can be affected by photo-bleaching. Such lifetime variation can cause critical error in many FLIM applications. If lifetime is changed by photo-bleaching, we can't measure accurately donor lifetime with acceptor. It is difficult to separate lifetime variation by photo-bleaching and FRET. For this reason we carry out the experiment to know influence of such fluorescence intensity loss on fluorescence lifetime.

Original languageEnglish
Title of host publication2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Volume3
ISBN (Electronic)9781467371094
DOIs
Publication statusPublished - 2016 Jan 7
Event11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015 - Busan, Korea, Republic of
Duration: 2015 Aug 242015 Aug 28

Other

Other11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015
CountryKorea, Republic of
CityBusan
Period15/8/2415/8/28

Fingerprint

Photobleaching
bleaching
Fluorescence
life (durability)
fluorescence
Dyes
dyes
causes
Experiments
decay

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

Hwang, W. S., Song, Y. S., & Kim, D. Y. (2016). Fluorescence lifetime determination of photo-bleaching correlation. In 2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015 (Vol. 3). [7376539] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/CLEOPR.2015.7376539
Hwang, Won Sang ; Song, Young Sik ; Kim, Dug Young. / Fluorescence lifetime determination of photo-bleaching correlation. 2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015. Vol. 3 Institute of Electrical and Electronics Engineers Inc., 2016.
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abstract = "FLIM is relatively stable about photo-bleaching effect. In case of dye lias multi-exponential decay property, fluorescence lifetime can be affected by photo-bleaching. Such lifetime variation can cause critical error in many FLIM applications. If lifetime is changed by photo-bleaching, we can't measure accurately donor lifetime with acceptor. It is difficult to separate lifetime variation by photo-bleaching and FRET. For this reason we carry out the experiment to know influence of such fluorescence intensity loss on fluorescence lifetime.",
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Hwang, WS, Song, YS & Kim, DY 2016, Fluorescence lifetime determination of photo-bleaching correlation. in 2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015. vol. 3, 7376539, Institute of Electrical and Electronics Engineers Inc., 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015, Busan, Korea, Republic of, 15/8/24. https://doi.org/10.1109/CLEOPR.2015.7376539

Fluorescence lifetime determination of photo-bleaching correlation. / Hwang, Won Sang; Song, Young Sik; Kim, Dug Young.

2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015. Vol. 3 Institute of Electrical and Electronics Engineers Inc., 2016. 7376539.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Song, Young Sik

AU - Kim, Dug Young

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N2 - FLIM is relatively stable about photo-bleaching effect. In case of dye lias multi-exponential decay property, fluorescence lifetime can be affected by photo-bleaching. Such lifetime variation can cause critical error in many FLIM applications. If lifetime is changed by photo-bleaching, we can't measure accurately donor lifetime with acceptor. It is difficult to separate lifetime variation by photo-bleaching and FRET. For this reason we carry out the experiment to know influence of such fluorescence intensity loss on fluorescence lifetime.

AB - FLIM is relatively stable about photo-bleaching effect. In case of dye lias multi-exponential decay property, fluorescence lifetime can be affected by photo-bleaching. Such lifetime variation can cause critical error in many FLIM applications. If lifetime is changed by photo-bleaching, we can't measure accurately donor lifetime with acceptor. It is difficult to separate lifetime variation by photo-bleaching and FRET. For this reason we carry out the experiment to know influence of such fluorescence intensity loss on fluorescence lifetime.

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Hwang WS, Song YS, Kim DY. Fluorescence lifetime determination of photo-bleaching correlation. In 2015 11th Conference on Lasers and Electro-Optics Pacific Rim, CLEO-PR 2015. Vol. 3. Institute of Electrical and Electronics Engineers Inc. 2016. 7376539 https://doi.org/10.1109/CLEOPR.2015.7376539