Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer

Wonsang Hwang, Jinwon Seo, Jun Ho Song, Dongeun Kim, Youngjae Won, In Hong Choi, Kyung Hwa Yoo, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI
EditorsDan V. Nicolau, Daniel L. Farkas, Robert C. Leif
PublisherSPIE
ISBN (Electronic)9781510614796
DOIs
Publication statusPublished - 2018 Jan 1
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018 - San Francisco, United States
Duration: 2018 Jan 292018 Jan 31

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10497
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018
CountryUnited States
CitySan Francisco
Period18/1/2918/1/31

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Hwang, W., Seo, J., Song, J. H., Kim, D., Won, Y., Choi, I. H., Yoo, K. H., & Kim, D. Y. (2018). Fluorescence lifetime microscopy for monitoring cell adhesion using metal induced energy transfer. In D. V. Nicolau, D. L. Farkas, & R. C. Leif (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI [104970E] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10497). SPIE. https://doi.org/10.1117/12.2289330