A precise control and a reliable monitoring tool for the adhesion properties of a cell are very important in atherosclerosis studies. If endothelial cells in contact with the intracellular membrane are not attached securely, low-density lipoprotein (LDL) particles can enter into the inner membrane. It is therefore necessary to measure conditions under which endothelial cell detachment occurs. When a cell is attached to a metal thin film, the lifetime of a fluorescence probe attached to the membrane of the cell is reduced by the metal-induced energy transfer (MIET). Fluorescence lifetime imaging microscopy (FLIM) is used to monitor the attachment condition of a cell to a metal surface using FRET. However, this requires high numerical aperture (NA) objective lens because axial confocal resolution must be smaller than the cell thickness. This requirement limits the field of view of the measurement specimen. In this study we provides a new method which can measure adhesion properties of endothelial cells even with a low NA objective lens by resolving two lifetime components in FLIM.
|Title of host publication||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI|
|Editors||Dan V. Nicolau, Daniel L. Farkas, Robert C. Leif|
|Publication status||Published - 2018|
|Event||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018 - San Francisco, United States|
Duration: 2018 Jan 29 → 2018 Jan 31
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Other||Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI 2018|
|Period||18/1/29 → 18/1/31|
Bibliographical noteFunding Information:
This work was financially supported by the National Research Foundation of Korea (NRF-2013R1A1A2062448) through the Basic Science Research Program, Global Frontier Project (CAMM-2014M3A6B3063712), the Ministry of Science and ICT of Korea, Technology Innovation Program (10062417), and the Ministry of Trade, Industry and Energy of Korea.
© 2018 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging