Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

Bavishna B. Praveen, Praveen C. Ashok, Michael Mazilu, Andrew Riches, Simon Herrington, Kishan Dholakia

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (∼30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.

Original languageEnglish
Article number077006
JournalJournal of Biomedical Optics
Volume17
Issue number7
DOIs
Publication statusPublished - 2012 Jul 1

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Raman spectroscopy
Fluorescence
retarding
Tissue
Wavelength
fluorescence
fibers
Fibers
probes
wavelengths
bones
adipose tissues
Bone
acquisition
chemical composition
signal to noise ratios
Raman scattering
Signal to noise ratio
optics
Raman spectra

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Praveen, Bavishna B. ; Ashok, Praveen C. ; Mazilu, Michael ; Riches, Andrew ; Herrington, Simon ; Dholakia, Kishan. / Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. In: Journal of Biomedical Optics. 2012 ; Vol. 17, No. 7.
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Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis. / Praveen, Bavishna B.; Ashok, Praveen C.; Mazilu, Michael; Riches, Andrew; Herrington, Simon; Dholakia, Kishan.

In: Journal of Biomedical Optics, Vol. 17, No. 7, 077006, 01.07.2012.

Research output: Contribution to journalArticle

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