Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis

Bavishna B. Praveen, Praveen C. Ashok, Michael Mazilu, Andrew Riches, Simon Herrington, Kishan Dholakia

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22 Citations (Scopus)


In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (∼30 s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.

Original languageEnglish
Article number077006
JournalJournal of Biomedical Optics
Issue number7
Publication statusPublished - 2012 Jul

Bibliographical note

Funding Information:
The work was funded by CR-UK/EPSRC/MRC/DoH (England) imaging programme. K. D. is a Royal Society-Wolfson Merit Award Holder.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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