Fluorescent iodized emulsion for pre- and intraoperative sentinel lymph node imaging: Validation in a preclinical model

Honsoul Kim, Sang Kil Lee, Yoo Min Kim, Eun Hye Lee, Soo Jeong Lim, Se Hoon Kim, Jaemoon Yang, Joon Seok Lim, Woo Jin Hyung

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Purpose: To validate the usefulness of a newly developed tracer for preoperative gastric sentinel lymph node (LN) (SLN) mapping and intraoperative navigation after a single preoperative submucosal injection in rat and beagle models. Materials and Methods: This study was approved by the Experimental Animal Ethical Committee of Yonsei University College of Medicine according to the eighth edition of the Guide for the Care and Use of Laboratory Animals published in 2011. An emulsion was developed that contained indocyanine green in iodized oil, which can be visualized with both computed tomography (CT) and near-infrared (NIR) optical imaging and has the property of delayed washout. This emulsion was injected into the footpad of rats (n = 6) and the gastric submucosa of beagles (n = 8). CT lymphography was performed. The degree of enhancement of popliteal LNs was measured in rats, and the enhancing LNs were identified and the degree of enhancement of the enhancing LNs was measured in beagles. Next, NIR imaging was performed in beagles during open, laparoscopic, and robotic surgery to identify LNs containing the fluorescent signals of indocyanine green. The enhanced LNs detected with CT lymphography and NIR imaging were matched to see if they corresponded. Results: Preoperative CT lymphography facilitated SLN mapping, and 26 SLNs were identified in eight beagles. NIR imaging enabled high-spatial-resolution visualization of both SLNs and the intervening lymphatic vessels and was useful for intraoperative SLN navigation. Conclusion: SLN mapping with fluorescent iodized oil emulsion is effective and feasible for both CT and NIR imaging.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalRadiology
Volume275
Issue number1
DOIs
Publication statusPublished - 2015 Apr 1

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

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