Formation of parkin aggregates and enhanced PINK1 accumulation during the pathogenesis of Parkinson's disease

Ji Won Um, Hyun Jung Park, Jihwan Song, Iksoo Jeon, Gwang Lee, philhyu Lee, Kwang Chul Chung

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13 Citations (Scopus)

Abstract

Parkinson's disease (PD) is a devastating neurodegenerative disease characterized by a distinct set of motor symptoms. Loss-of-function mutations in PTEN-induced kinase 1 (PINK1) or parkin have been linked to early-onset autosomal recessive forms of familial PD. We have recently shown that parkin (an E3 ubiquitin ligase) and PINK1 (a serine/threonine kinase) affect one other's stability, solubility, and tendency to form cytoprotective aggresomes (Um et al., 2009, [16]). Here we validated the functional relevance of this mutual interaction under pathologic PD conditions, by investigating the changes of expression and solubility of these factors in response to PD-linked toxins. Consistent with our previous cell culture data, exposure of human dopaminergic neuroblastoma SH-SY5Y cells to PD-linked toxins (1-methyl-4-phenylpyridinium ion, 6-hydroxydopamine, or MG132) reduced Nonidet P-40-soluble parkin levels and induced PINK1 accumulation. Consistent with our previous findings from parkin knockout mice, rat models of PD (6-hydroxydopamine-, rotenone-, or MG132-induced PD) were also associated with an increase in soluble and insoluble PINK1 levels as well as enhanced formation of parkin aggregates. These findings suggest that both PINK1 and parkin play important roles in regulating the formation of Lewy bodies during the pathogenesis of sporadic and familial PD.

Original languageEnglish
Pages (from-to)824-828
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number4
DOIs
Publication statusPublished - 2010 Mar 19

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Parkinson Disease
Phosphotransferases
Oxidopamine
Solubility
1-Methyl-4-phenylpyridinium
Lewy Bodies
Rotenone
Neurodegenerative diseases
Ubiquitin-Protein Ligases
Protein-Serine-Threonine Kinases
Parkinsonian Disorders
Neuroblastoma
Knockout Mice
Neurodegenerative Diseases
Cell culture
Cell Culture Techniques
Rats
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Parkinson's disease (PD) is a devastating neurodegenerative disease characterized by a distinct set of motor symptoms. Loss-of-function mutations in PTEN-induced kinase 1 (PINK1) or parkin have been linked to early-onset autosomal recessive forms of familial PD. We have recently shown that parkin (an E3 ubiquitin ligase) and PINK1 (a serine/threonine kinase) affect one other's stability, solubility, and tendency to form cytoprotective aggresomes (Um et al., 2009, [16]). Here we validated the functional relevance of this mutual interaction under pathologic PD conditions, by investigating the changes of expression and solubility of these factors in response to PD-linked toxins. Consistent with our previous cell culture data, exposure of human dopaminergic neuroblastoma SH-SY5Y cells to PD-linked toxins (1-methyl-4-phenylpyridinium ion, 6-hydroxydopamine, or MG132) reduced Nonidet P-40-soluble parkin levels and induced PINK1 accumulation. Consistent with our previous findings from parkin knockout mice, rat models of PD (6-hydroxydopamine-, rotenone-, or MG132-induced PD) were also associated with an increase in soluble and insoluble PINK1 levels as well as enhanced formation of parkin aggregates. These findings suggest that both PINK1 and parkin play important roles in regulating the formation of Lewy bodies during the pathogenesis of sporadic and familial PD.",
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Formation of parkin aggregates and enhanced PINK1 accumulation during the pathogenesis of Parkinson's disease. / Um, Ji Won; Park, Hyun Jung; Song, Jihwan; Jeon, Iksoo; Lee, Gwang; Lee, philhyu; Chung, Kwang Chul.

In: Biochemical and Biophysical Research Communications, Vol. 393, No. 4, 19.03.2010, p. 824-828.

Research output: Contribution to journalArticle

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