Formation of spacing pattern and morphogenesis of chick feather buds is regulated by cytoskeletal structures

Jae Young Kim, Sung Won Cho, Wu Chul Song, Min Jung Lee, Jinglei Cai, Seung Ho Ohk, Hee Kyung Song, Alexander Degan, Han Sung Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Chick feather buds develop sequentially in a hexagonal array. Each feather bud develops with anterior posterior polarity, which is thought to develop in response to signals derived from specialized regions of mesenchymal condensation and epithelial thickening. These developmental processes are performed by cellular mechanisms, such as cell proliferation and migration, which occur during chick feather bud development. In order to understand the mechanisms regulating the formation of mesenchymal condensation and their role in feather bud development, we explanted chick dorsal skin at stage HH29+ with cytochalasin D, which inhibits cytoskeletal formation. We show that the aggregation of mesenchymal cells can be prevented by cytochalasin D treatment in a concentration-dependent manner. Subsequently, cytochalasin D disrupts the spacing pattern and inhibits feather bud axis formation as well. In addition, expression patterns of Bmp-4 and Msx-2, key molecules for early feather bud development, were disturbed by cytochalasin D treatment. Our results fully indicate that both the cytoskeletal structure and cell activity via gene regulation are of fundamental importance in mesenchymal condensation leading to proper morphogenesis of feather bud and spacing pattern formation.

Original languageEnglish
Pages (from-to)240-248
Number of pages9
JournalDifferentiation
Volume73
Issue number5
DOIs
Publication statusPublished - 2005 Jun

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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