Formation of wheat protein bodies

Involvement of the Golgi apparatus in gliadin transport

Woo Taek Kim, Vincent R. Franceschi, Hari B. Krishnan, Thomas W. Okita

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Developing wheat (Triticum aestivum L.) endosperm was examined using ultrathin sections prepared from tissues harvested at 5, 9, 16 and 25 d after flowering. Protein bodies were evident by 9 d and displayed a variety of membranous structures and inclusions. The Golgi apparatus was a prominent organelle at all stages, and by 9 d was associated with small electron-dense inclusions. By immunocytochemical techniques, gliadin (wheat prolamine) was localized within these vesicles and in homogeneous regions of protein bodies, but not in the lumen of the rough endoplasmic reticulum. The protein bodies appear to enlarge by fusion of smaller protein bodies resulting in larger, irregular-shaped organelles. The affinity of the Golgi-derived vesicles for gliadin-specific probes during the period of maximal storage-protein synthesis and deposition indicates that this organelle includes the bulk, if not all, of the gliadin produced. The involvement of the Golgi apparatus in the packaging of gliadins into protein bodies indicates a pathway which differs from the mode of prolamine deposition in other cereals such as maize, rice and sorghum, and resembles the mechanism employed for the storage of rice glutelin and legume globulins.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalPlanta
Volume176
Issue number2
DOIs
Publication statusPublished - 1988 Nov 1

Fingerprint

Gliadin
wheat protein
protein bodies
gliadin
Golgi Apparatus
Golgi apparatus
Triticum
organelles
Organelles
Phenylpropanolamine
Proteins
rice
glutelins
protein deposition
wheat
rough endoplasmic reticulum
storage proteins
Body Regions
Endosperm
Sorghum

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Kim, Woo Taek ; Franceschi, Vincent R. ; Krishnan, Hari B. ; Okita, Thomas W. / Formation of wheat protein bodies : Involvement of the Golgi apparatus in gliadin transport. In: Planta. 1988 ; Vol. 176, No. 2. pp. 173-182.
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Formation of wheat protein bodies : Involvement of the Golgi apparatus in gliadin transport. / Kim, Woo Taek; Franceschi, Vincent R.; Krishnan, Hari B.; Okita, Thomas W.

In: Planta, Vol. 176, No. 2, 01.11.1988, p. 173-182.

Research output: Contribution to journalArticle

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