Four exact GES variants used for identification of GES-type extended-spectrum β-lactamases with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing

Hun Lee Jung, Jin Bae Seung, Jin Lee Jae, Kwon Bae Il, Seokhoon Jeong, Suk Sohn Eui, Seon Lee Yeong, Hee Lee Sang

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

In our proposed nomenclature system including GES-type (Guiana-Extended- Spectrum β-lactamase) or IBC-type (Integron-Borne Cephalosporinase) extended-spectrum β-lactamases (ESBLs), GES-2 has an amino acid substitution of G170N, GES-3 has two M62T and E104K substitutions, GES-4 has three M62T, E104K and G170S substitutions, GES-5 has a G170S substitution, GES-6 has two F104K and G170S substitutions, GES-7 (IBC-1) has a G104K substitution, GES-8 (IBC-2) has a A125L substitution and GES-9 has a G243S substitution, compared to the sequence of GES-1. This proposed nomenclature update would not confuse microbiologists studying GES-type ESBLs, fundamentally preventing misleading nomenclature of these antibiotic resistance genes. The definitive renaming of GES/IBC-type ESBLs can help also some researchers to conectly designate new GES-type ESBLs such as novel enzymes identified from some nationwide surveys. In order to detect rapidly and exactly GES-type ESBLs with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing, we also proposed here the new four key amino acid substitutions and four GES-type variants as follows: (i) GES-1 able to hydrolyze ceftazidime, (ii) GES-4 able to hydrolyze ceftazidime, cefoxitin and imipenem and able to show inhibitor-resistance, (iii) GES-5 able to hydrolyze ceftazidime, cefoxitin and imipenem and (iv) GES-9 able to hydrolyze ceftazidime and aztreonam.

Original languageEnglish
Pages (from-to)486-490
Number of pages5
JournalResearch Journal of Microbiology
Volume2
Issue number5
Publication statusPublished - 2007 Aug 21

Fingerprint

Ceftazidime
Hydrolysis
hydrolysis
Terminology
imipenem
Cefoxitin
Imipenem
amino acid substitution
Amino Acid Substitution
aztreonam
Cephalosporinase
Integrons
Aztreonam
beta-lactamase
national surveys
Microbial Drug Resistance
antibiotic resistance
researchers
Research Personnel
ceftazidime

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Infectious Diseases

Cite this

Jung, Hun Lee ; Seung, Jin Bae ; Jae, Jin Lee ; Il, Kwon Bae ; Jeong, Seokhoon ; Eui, Suk Sohn ; Yeong, Seon Lee ; Sang, Hee Lee. / Four exact GES variants used for identification of GES-type extended-spectrum β-lactamases with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing. In: Research Journal of Microbiology. 2007 ; Vol. 2, No. 5. pp. 486-490.
@article{47e2bf3f8a924595a9b9792f96f9c351,
title = "Four exact GES variants used for identification of GES-type extended-spectrum β-lactamases with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing",
abstract = "In our proposed nomenclature system including GES-type (Guiana-Extended- Spectrum β-lactamase) or IBC-type (Integron-Borne Cephalosporinase) extended-spectrum β-lactamases (ESBLs), GES-2 has an amino acid substitution of G170N, GES-3 has two M62T and E104K substitutions, GES-4 has three M62T, E104K and G170S substitutions, GES-5 has a G170S substitution, GES-6 has two F104K and G170S substitutions, GES-7 (IBC-1) has a G104K substitution, GES-8 (IBC-2) has a A125L substitution and GES-9 has a G243S substitution, compared to the sequence of GES-1. This proposed nomenclature update would not confuse microbiologists studying GES-type ESBLs, fundamentally preventing misleading nomenclature of these antibiotic resistance genes. The definitive renaming of GES/IBC-type ESBLs can help also some researchers to conectly designate new GES-type ESBLs such as novel enzymes identified from some nationwide surveys. In order to detect rapidly and exactly GES-type ESBLs with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing, we also proposed here the new four key amino acid substitutions and four GES-type variants as follows: (i) GES-1 able to hydrolyze ceftazidime, (ii) GES-4 able to hydrolyze ceftazidime, cefoxitin and imipenem and able to show inhibitor-resistance, (iii) GES-5 able to hydrolyze ceftazidime, cefoxitin and imipenem and (iv) GES-9 able to hydrolyze ceftazidime and aztreonam.",
author = "Jung, {Hun Lee} and Seung, {Jin Bae} and Jae, {Jin Lee} and Il, {Kwon Bae} and Seokhoon Jeong and Eui, {Suk Sohn} and Yeong, {Seon Lee} and Sang, {Hee Lee}",
year = "2007",
month = "8",
day = "21",
language = "English",
volume = "2",
pages = "486--490",
journal = "International Journal of Soil Science",
issn = "1816-4935",
publisher = "Academic Journals Inc.",
number = "5",

}

Four exact GES variants used for identification of GES-type extended-spectrum β-lactamases with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing. / Jung, Hun Lee; Seung, Jin Bae; Jae, Jin Lee; Il, Kwon Bae; Jeong, Seokhoon; Eui, Suk Sohn; Yeong, Seon Lee; Sang, Hee Lee.

In: Research Journal of Microbiology, Vol. 2, No. 5, 21.08.2007, p. 486-490.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Four exact GES variants used for identification of GES-type extended-spectrum β-lactamases with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing

AU - Jung, Hun Lee

AU - Seung, Jin Bae

AU - Jae, Jin Lee

AU - Il, Kwon Bae

AU - Jeong, Seokhoon

AU - Eui, Suk Sohn

AU - Yeong, Seon Lee

AU - Sang, Hee Lee

PY - 2007/8/21

Y1 - 2007/8/21

N2 - In our proposed nomenclature system including GES-type (Guiana-Extended- Spectrum β-lactamase) or IBC-type (Integron-Borne Cephalosporinase) extended-spectrum β-lactamases (ESBLs), GES-2 has an amino acid substitution of G170N, GES-3 has two M62T and E104K substitutions, GES-4 has three M62T, E104K and G170S substitutions, GES-5 has a G170S substitution, GES-6 has two F104K and G170S substitutions, GES-7 (IBC-1) has a G104K substitution, GES-8 (IBC-2) has a A125L substitution and GES-9 has a G243S substitution, compared to the sequence of GES-1. This proposed nomenclature update would not confuse microbiologists studying GES-type ESBLs, fundamentally preventing misleading nomenclature of these antibiotic resistance genes. The definitive renaming of GES/IBC-type ESBLs can help also some researchers to conectly designate new GES-type ESBLs such as novel enzymes identified from some nationwide surveys. In order to detect rapidly and exactly GES-type ESBLs with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing, we also proposed here the new four key amino acid substitutions and four GES-type variants as follows: (i) GES-1 able to hydrolyze ceftazidime, (ii) GES-4 able to hydrolyze ceftazidime, cefoxitin and imipenem and able to show inhibitor-resistance, (iii) GES-5 able to hydrolyze ceftazidime, cefoxitin and imipenem and (iv) GES-9 able to hydrolyze ceftazidime and aztreonam.

AB - In our proposed nomenclature system including GES-type (Guiana-Extended- Spectrum β-lactamase) or IBC-type (Integron-Borne Cephalosporinase) extended-spectrum β-lactamases (ESBLs), GES-2 has an amino acid substitution of G170N, GES-3 has two M62T and E104K substitutions, GES-4 has three M62T, E104K and G170S substitutions, GES-5 has a G170S substitution, GES-6 has two F104K and G170S substitutions, GES-7 (IBC-1) has a G104K substitution, GES-8 (IBC-2) has a A125L substitution and GES-9 has a G243S substitution, compared to the sequence of GES-1. This proposed nomenclature update would not confuse microbiologists studying GES-type ESBLs, fundamentally preventing misleading nomenclature of these antibiotic resistance genes. The definitive renaming of GES/IBC-type ESBLs can help also some researchers to conectly designate new GES-type ESBLs such as novel enzymes identified from some nationwide surveys. In order to detect rapidly and exactly GES-type ESBLs with different hydrolysis profiles and inhibitor-resistance patterns by pyrosequencing, we also proposed here the new four key amino acid substitutions and four GES-type variants as follows: (i) GES-1 able to hydrolyze ceftazidime, (ii) GES-4 able to hydrolyze ceftazidime, cefoxitin and imipenem and able to show inhibitor-resistance, (iii) GES-5 able to hydrolyze ceftazidime, cefoxitin and imipenem and (iv) GES-9 able to hydrolyze ceftazidime and aztreonam.

UR - http://www.scopus.com/inward/record.url?scp=34547885082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547885082&partnerID=8YFLogxK

M3 - Review article

VL - 2

SP - 486

EP - 490

JO - International Journal of Soil Science

JF - International Journal of Soil Science

SN - 1816-4935

IS - 5

ER -