Four-week individual caging of male ICR mice alters body composition without change in body mass

Jisu Shin, Jiwan Woo, Yakdol Cho, Yoon Hee Choi, Naewoo Neo Shin, YoungSoo Kim

Research output: Contribution to journalArticle

Abstract

Understanding the physiological implications of caging conditions for mice is crucial in improving the replicability and reliability of animal research. Individual caging of mice is known to alter mouse psychology, such as triggering depression-like symptoms in mice, suggesting that caging conditions could have negative effects on mice. Therefore, we hypothesized that individual caging could affect the physical composition of outbred mice. To investigate this, dual X-ray absorptiometry (DXA) was used to compare the mass, bone mineral content (BMC), bone mineral density (BMD), lean tissue percentage and fat tissue percentage between group and individual caged mice. We also conducted open field test to compare mouse activities in different caging conditions. Our results showed significantly reduced BMD and lean tissue percentage and significantly increased fat tissue percentage in individually-caged male mice. Furthermore, there were no differences in body mass and activity between the grouped and individual mice, suggesting that these physical alterations were not induced by group-related activity. In this study, we conclude that individual caging could alter the body composition of mice without affecting external morphology.

Original languageEnglish
Article number1331
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Inbred ICR Mouse
Body Composition
Bone Density
Fats
Photon Absorptiometry
Depression
Psychology

All Science Journal Classification (ASJC) codes

  • General

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Shin, Jisu ; Woo, Jiwan ; Cho, Yakdol ; Choi, Yoon Hee ; Shin, Naewoo Neo ; Kim, YoungSoo. / Four-week individual caging of male ICR mice alters body composition without change in body mass. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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Four-week individual caging of male ICR mice alters body composition without change in body mass. / Shin, Jisu; Woo, Jiwan; Cho, Yakdol; Choi, Yoon Hee; Shin, Naewoo Neo; Kim, YoungSoo.

In: Scientific reports, Vol. 8, No. 1, 1331, 01.12.2018.

Research output: Contribution to journalArticle

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