Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells

Rae Kwon Kim, Yongjoon Suh, Yan Hong Cui, Eunji Hwang, Eun Jung Lim, Ki Chun Yoo, Ga Haeng Lee, Joo Mi Yi, Seok-Gu Kang, Su Jae Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Glioblastoma remains an incurable brain disease due to the prevalence of its recurrence. Considerable evidence suggests that glioma stem-like cells are responsible for glioma relapse after treatment, which commonly involves ionizing radiation. Here, we found that fractionated ionizing radiation (2 Gy/day for 3 days) induced glioma stem-like cell expansion and resistance to anticancer treatment such as cisplatin (50 μM) or taxol (500 nM), or by ionizing radiation (10 Gy) in both glioma cell lines (U87, U373) and patient-derived glioma cells. Of note, concomitant increase of nitric oxide production occurred with the radiation-induced increase of the glioma stem-like cell population through upregulation of inducible nitric oxide synthase (iNOS). In line with this observation, downregulation of iNOS effectively reduced the glioma stem-like cell population and decreased resistance to anticancer treatment. Collectively, our results suggest that targeting iNOS in combination with ionizing radiation might increase the efficacy of radiotherapy for glioma treatment.

Original languageEnglish
Pages (from-to)1172-1177
Number of pages6
JournalCancer Science
Volume104
Issue number9
DOIs
Publication statusPublished - 2013 Sep 1

Fingerprint

Glioma
Nitric Oxide
Stem Cells
Radiation
Ionizing Radiation
Nitric Oxide Synthase Type II
Recurrence
Brain Diseases
Therapeutics
Glioblastoma
Paclitaxel
Cisplatin
Population
Up-Regulation
Radiotherapy
Down-Regulation
Cell Line

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Kim, R. K., Suh, Y., Cui, Y. H., Hwang, E., Lim, E. J., Yoo, K. C., ... Lee, S. J. (2013). Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells. Cancer Science, 104(9), 1172-1177. https://doi.org/10.1111/cas.12207
Kim, Rae Kwon ; Suh, Yongjoon ; Cui, Yan Hong ; Hwang, Eunji ; Lim, Eun Jung ; Yoo, Ki Chun ; Lee, Ga Haeng ; Yi, Joo Mi ; Kang, Seok-Gu ; Lee, Su Jae. / Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells. In: Cancer Science. 2013 ; Vol. 104, No. 9. pp. 1172-1177.
@article{5832c9bddecb49278e365ae0e261696e,
title = "Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells",
abstract = "Glioblastoma remains an incurable brain disease due to the prevalence of its recurrence. Considerable evidence suggests that glioma stem-like cells are responsible for glioma relapse after treatment, which commonly involves ionizing radiation. Here, we found that fractionated ionizing radiation (2 Gy/day for 3 days) induced glioma stem-like cell expansion and resistance to anticancer treatment such as cisplatin (50 μM) or taxol (500 nM), or by ionizing radiation (10 Gy) in both glioma cell lines (U87, U373) and patient-derived glioma cells. Of note, concomitant increase of nitric oxide production occurred with the radiation-induced increase of the glioma stem-like cell population through upregulation of inducible nitric oxide synthase (iNOS). In line with this observation, downregulation of iNOS effectively reduced the glioma stem-like cell population and decreased resistance to anticancer treatment. Collectively, our results suggest that targeting iNOS in combination with ionizing radiation might increase the efficacy of radiotherapy for glioma treatment.",
author = "Kim, {Rae Kwon} and Yongjoon Suh and Cui, {Yan Hong} and Eunji Hwang and Lim, {Eun Jung} and Yoo, {Ki Chun} and Lee, {Ga Haeng} and Yi, {Joo Mi} and Seok-Gu Kang and Lee, {Su Jae}",
year = "2013",
month = "9",
day = "1",
doi = "10.1111/cas.12207",
language = "English",
volume = "104",
pages = "1172--1177",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "9",

}

Kim, RK, Suh, Y, Cui, YH, Hwang, E, Lim, EJ, Yoo, KC, Lee, GH, Yi, JM, Kang, S-G & Lee, SJ 2013, 'Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells', Cancer Science, vol. 104, no. 9, pp. 1172-1177. https://doi.org/10.1111/cas.12207

Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells. / Kim, Rae Kwon; Suh, Yongjoon; Cui, Yan Hong; Hwang, Eunji; Lim, Eun Jung; Yoo, Ki Chun; Lee, Ga Haeng; Yi, Joo Mi; Kang, Seok-Gu; Lee, Su Jae.

In: Cancer Science, Vol. 104, No. 9, 01.09.2013, p. 1172-1177.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fractionated radiation-induced nitric oxide promotes expansion of glioma stem-like cells

AU - Kim, Rae Kwon

AU - Suh, Yongjoon

AU - Cui, Yan Hong

AU - Hwang, Eunji

AU - Lim, Eun Jung

AU - Yoo, Ki Chun

AU - Lee, Ga Haeng

AU - Yi, Joo Mi

AU - Kang, Seok-Gu

AU - Lee, Su Jae

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Glioblastoma remains an incurable brain disease due to the prevalence of its recurrence. Considerable evidence suggests that glioma stem-like cells are responsible for glioma relapse after treatment, which commonly involves ionizing radiation. Here, we found that fractionated ionizing radiation (2 Gy/day for 3 days) induced glioma stem-like cell expansion and resistance to anticancer treatment such as cisplatin (50 μM) or taxol (500 nM), or by ionizing radiation (10 Gy) in both glioma cell lines (U87, U373) and patient-derived glioma cells. Of note, concomitant increase of nitric oxide production occurred with the radiation-induced increase of the glioma stem-like cell population through upregulation of inducible nitric oxide synthase (iNOS). In line with this observation, downregulation of iNOS effectively reduced the glioma stem-like cell population and decreased resistance to anticancer treatment. Collectively, our results suggest that targeting iNOS in combination with ionizing radiation might increase the efficacy of radiotherapy for glioma treatment.

AB - Glioblastoma remains an incurable brain disease due to the prevalence of its recurrence. Considerable evidence suggests that glioma stem-like cells are responsible for glioma relapse after treatment, which commonly involves ionizing radiation. Here, we found that fractionated ionizing radiation (2 Gy/day for 3 days) induced glioma stem-like cell expansion and resistance to anticancer treatment such as cisplatin (50 μM) or taxol (500 nM), or by ionizing radiation (10 Gy) in both glioma cell lines (U87, U373) and patient-derived glioma cells. Of note, concomitant increase of nitric oxide production occurred with the radiation-induced increase of the glioma stem-like cell population through upregulation of inducible nitric oxide synthase (iNOS). In line with this observation, downregulation of iNOS effectively reduced the glioma stem-like cell population and decreased resistance to anticancer treatment. Collectively, our results suggest that targeting iNOS in combination with ionizing radiation might increase the efficacy of radiotherapy for glioma treatment.

UR - http://www.scopus.com/inward/record.url?scp=84883459098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883459098&partnerID=8YFLogxK

U2 - 10.1111/cas.12207

DO - 10.1111/cas.12207

M3 - Article

VL - 104

SP - 1172

EP - 1177

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 9

ER -