Frequency- and spectrally-encoded confocal microscopy

Jaehyun Hwang, Soocheol Kim, Jung Heo, Donghak Lee, Suho Ryu, Chulmin Joo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We describe a three-dimensional microscopy technique based on spectral and frequency encoding. The method employs a wavelength-swept laser to illuminate a specimen with a spectrally-dispersed line focus that sweeps over the specimen in time. The spatial information along each spectral line is further mapped into different modulation frequencies. Spectrally-resolved detection and subsequent Fourier analysis of the backscattered light from the specimen therefore enable high-speed, scanner-free imaging of the specimen with a single-element photodetector. Highcontrast, three-dimensional imaging capability of this method is demonstrated by presenting images of various materials and biological specimens.

Original languageEnglish
Pages (from-to)5809-5821
Number of pages13
JournalOptics Express
Volume23
Issue number5
DOIs
Publication statusPublished - 2015 Jan 1

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microscopy
Fourier analysis
frequency modulation
scanners
line spectra
photometers
coding
high speed
wavelengths
lasers

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Hwang, J., Kim, S., Heo, J., Lee, D., Ryu, S., & Joo, C. (2015). Frequency- and spectrally-encoded confocal microscopy. Optics Express, 23(5), 5809-5821. https://doi.org/10.1364/OE.23.005809
Hwang, Jaehyun ; Kim, Soocheol ; Heo, Jung ; Lee, Donghak ; Ryu, Suho ; Joo, Chulmin. / Frequency- and spectrally-encoded confocal microscopy. In: Optics Express. 2015 ; Vol. 23, No. 5. pp. 5809-5821.
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Hwang, J, Kim, S, Heo, J, Lee, D, Ryu, S & Joo, C 2015, 'Frequency- and spectrally-encoded confocal microscopy', Optics Express, vol. 23, no. 5, pp. 5809-5821. https://doi.org/10.1364/OE.23.005809

Frequency- and spectrally-encoded confocal microscopy. / Hwang, Jaehyun; Kim, Soocheol; Heo, Jung; Lee, Donghak; Ryu, Suho; Joo, Chulmin.

In: Optics Express, Vol. 23, No. 5, 01.01.2015, p. 5809-5821.

Research output: Contribution to journalArticle

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