Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients: Detection by the Elecsys ® HBsAg II assay

Hyon Suk Kim, Xinyue Chen, Min Xu, Cunling Yan, Yali Liu, Haohui Deng, Bui Huu Hoang, Pham Thi Thu Thuy, Terry Wang, Yiwen Yan, Zhen Zeng, Mikael Gencay, Gaston Westergaard, Stephan Pabinger, Albert Kriegner, Markus Nauck, Anja Seffner, Peter Gohl, Kirsten Hübner, Wolfgang E. Kaminski

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant ‘a’ determinant region, which vary by ethnographic region. Objective: We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys ® HBsAg II Qualitative assay. Study design: We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam). HBsAg mutations were detected and sequenced using highly sensitive ultra-deep sequencing and compared between the first (amino acids 124–137) and second (amino acids 139–147) loops of the ‘a’ determinant region using the Elecsys ® HBsAg II Qualitative assay. Results: Overall, 237 distinct amino acid mutations in the major hydrophilic region were identified; mutations were present in 660 of 898 HBV-infected patient samples (73.5%). Within the pool of 237 distinct mutations, the majority of the amino acid mutations were found in HBV genotype C (64.8%). We identified 25 previously unknown distinct mutations, mostly prevalent in genotype C-infected Korean patients (n = 18) followed by Chinese (n = 12) patients. All 898 samples were correctly identified by the Elecsys ® HBsAg II Qualitative assay. Conclusions: We observed 237 distinct (including 25 novel) mutations, demonstrating the complexity of HBsAg variants in HBV-infected East- and Southeast Asian patients. The Elecsys ® HBsAg II Qualitative assay can reliably detect HBV-positive samples and is suitable for routine diagnostic use in East and Southeast Asia.

Original languageEnglish
Pages (from-to)48-56
Number of pages9
JournalJournal of Clinical Virology
Volume103
DOIs
Publication statusPublished - 2018 Jun

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Hepatitis B Surface Antigens
Hepatitis B virus
Genotype
Mutation
Amino Acids
High-Throughput Nucleotide Sequencing
Immunodominant Epitopes
Southeastern Asia
Far East
Vietnam
Virus Diseases
Korea
China

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Kim, Hyon Suk ; Chen, Xinyue ; Xu, Min ; Yan, Cunling ; Liu, Yali ; Deng, Haohui ; Hoang, Bui Huu ; Thuy, Pham Thi Thu ; Wang, Terry ; Yan, Yiwen ; Zeng, Zhen ; Gencay, Mikael ; Westergaard, Gaston ; Pabinger, Stephan ; Kriegner, Albert ; Nauck, Markus ; Seffner, Anja ; Gohl, Peter ; Hübner, Kirsten ; Kaminski, Wolfgang E. / Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients : Detection by the Elecsys ® HBsAg II assay. In: Journal of Clinical Virology. 2018 ; Vol. 103. pp. 48-56.
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title = "Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients: Detection by the Elecsys {\circledR} HBsAg II assay",
abstract = "Background: To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant ‘a’ determinant region, which vary by ethnographic region. Objective: We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys {\circledR} HBsAg II Qualitative assay. Study design: We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam). HBsAg mutations were detected and sequenced using highly sensitive ultra-deep sequencing and compared between the first (amino acids 124–137) and second (amino acids 139–147) loops of the ‘a’ determinant region using the Elecsys {\circledR} HBsAg II Qualitative assay. Results: Overall, 237 distinct amino acid mutations in the major hydrophilic region were identified; mutations were present in 660 of 898 HBV-infected patient samples (73.5{\%}). Within the pool of 237 distinct mutations, the majority of the amino acid mutations were found in HBV genotype C (64.8{\%}). We identified 25 previously unknown distinct mutations, mostly prevalent in genotype C-infected Korean patients (n = 18) followed by Chinese (n = 12) patients. All 898 samples were correctly identified by the Elecsys {\circledR} HBsAg II Qualitative assay. Conclusions: We observed 237 distinct (including 25 novel) mutations, demonstrating the complexity of HBsAg variants in HBV-infected East- and Southeast Asian patients. The Elecsys {\circledR} HBsAg II Qualitative assay can reliably detect HBV-positive samples and is suitable for routine diagnostic use in East and Southeast Asia.",
author = "Kim, {Hyon Suk} and Xinyue Chen and Min Xu and Cunling Yan and Yali Liu and Haohui Deng and Hoang, {Bui Huu} and Thuy, {Pham Thi Thu} and Terry Wang and Yiwen Yan and Zhen Zeng and Mikael Gencay and Gaston Westergaard and Stephan Pabinger and Albert Kriegner and Markus Nauck and Anja Seffner and Peter Gohl and Kirsten H{\"u}bner and Kaminski, {Wolfgang E.}",
year = "2018",
month = "6",
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Kim, HS, Chen, X, Xu, M, Yan, C, Liu, Y, Deng, H, Hoang, BH, Thuy, PTT, Wang, T, Yan, Y, Zeng, Z, Gencay, M, Westergaard, G, Pabinger, S, Kriegner, A, Nauck, M, Seffner, A, Gohl, P, Hübner, K & Kaminski, WE 2018, 'Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients: Detection by the Elecsys ® HBsAg II assay', Journal of Clinical Virology, vol. 103, pp. 48-56. https://doi.org/10.1016/j.jcv.2018.04.005

Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients : Detection by the Elecsys ® HBsAg II assay. / Kim, Hyon Suk; Chen, Xinyue; Xu, Min; Yan, Cunling; Liu, Yali; Deng, Haohui; Hoang, Bui Huu; Thuy, Pham Thi Thu; Wang, Terry; Yan, Yiwen; Zeng, Zhen; Gencay, Mikael; Westergaard, Gaston; Pabinger, Stephan; Kriegner, Albert; Nauck, Markus; Seffner, Anja; Gohl, Peter; Hübner, Kirsten; Kaminski, Wolfgang E.

In: Journal of Clinical Virology, Vol. 103, 06.2018, p. 48-56.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients

T2 - Detection by the Elecsys ® HBsAg II assay

AU - Kim, Hyon Suk

AU - Chen, Xinyue

AU - Xu, Min

AU - Yan, Cunling

AU - Liu, Yali

AU - Deng, Haohui

AU - Hoang, Bui Huu

AU - Thuy, Pham Thi Thu

AU - Wang, Terry

AU - Yan, Yiwen

AU - Zeng, Zhen

AU - Gencay, Mikael

AU - Westergaard, Gaston

AU - Pabinger, Stephan

AU - Kriegner, Albert

AU - Nauck, Markus

AU - Seffner, Anja

AU - Gohl, Peter

AU - Hübner, Kirsten

AU - Kaminski, Wolfgang E.

PY - 2018/6

Y1 - 2018/6

N2 - Background: To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant ‘a’ determinant region, which vary by ethnographic region. Objective: We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys ® HBsAg II Qualitative assay. Study design: We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam). HBsAg mutations were detected and sequenced using highly sensitive ultra-deep sequencing and compared between the first (amino acids 124–137) and second (amino acids 139–147) loops of the ‘a’ determinant region using the Elecsys ® HBsAg II Qualitative assay. Results: Overall, 237 distinct amino acid mutations in the major hydrophilic region were identified; mutations were present in 660 of 898 HBV-infected patient samples (73.5%). Within the pool of 237 distinct mutations, the majority of the amino acid mutations were found in HBV genotype C (64.8%). We identified 25 previously unknown distinct mutations, mostly prevalent in genotype C-infected Korean patients (n = 18) followed by Chinese (n = 12) patients. All 898 samples were correctly identified by the Elecsys ® HBsAg II Qualitative assay. Conclusions: We observed 237 distinct (including 25 novel) mutations, demonstrating the complexity of HBsAg variants in HBV-infected East- and Southeast Asian patients. The Elecsys ® HBsAg II Qualitative assay can reliably detect HBV-positive samples and is suitable for routine diagnostic use in East and Southeast Asia.

AB - Background: To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant ‘a’ determinant region, which vary by ethnographic region. Objective: We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys ® HBsAg II Qualitative assay. Study design: We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam). HBsAg mutations were detected and sequenced using highly sensitive ultra-deep sequencing and compared between the first (amino acids 124–137) and second (amino acids 139–147) loops of the ‘a’ determinant region using the Elecsys ® HBsAg II Qualitative assay. Results: Overall, 237 distinct amino acid mutations in the major hydrophilic region were identified; mutations were present in 660 of 898 HBV-infected patient samples (73.5%). Within the pool of 237 distinct mutations, the majority of the amino acid mutations were found in HBV genotype C (64.8%). We identified 25 previously unknown distinct mutations, mostly prevalent in genotype C-infected Korean patients (n = 18) followed by Chinese (n = 12) patients. All 898 samples were correctly identified by the Elecsys ® HBsAg II Qualitative assay. Conclusions: We observed 237 distinct (including 25 novel) mutations, demonstrating the complexity of HBsAg variants in HBV-infected East- and Southeast Asian patients. The Elecsys ® HBsAg II Qualitative assay can reliably detect HBV-positive samples and is suitable for routine diagnostic use in East and Southeast Asia.

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U2 - 10.1016/j.jcv.2018.04.005

DO - 10.1016/j.jcv.2018.04.005

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JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

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