Abstract
Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Fucosterol, which is isolated from marine brown algae, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of fucosterol on photoaging and investigated its mechanisms of action in UV-irradiated immortalized human keratinocytes (HaCaT) by enzyme-linked immunosorbent assay, semi-quantitative reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that fucosterol attenuated UV-induced MMP and inflammatory cytokine expression by deactivating mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Fucosterol also increased type-I procollagen and antioxidant enzyme expression. Taken together, fucosterol regulates the expression of MMPs and type-I procollagen in UV-irradiated HaCaT by modulating MAPK, suggesting it as a potential candidate for prevention and treatment of skin aging.
| Original language | English |
|---|---|
| Pages (from-to) | 911-918 |
| Number of pages | 8 |
| Journal | Photochemistry and Photobiology |
| Volume | 89 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 Jul 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Physical and Theoretical Chemistry
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Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells. / Kim, Myung Suk; Oh, Ga Hui; Kim, Min Ji; Hwang, Jae-Kwan.
In: Photochemistry and Photobiology, Vol. 89, No. 4, 01.07.2013, p. 911-918.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells
AU - Kim, Myung Suk
AU - Oh, Ga Hui
AU - Kim, Min Ji
AU - Hwang, Jae-Kwan
PY - 2013/7/1
Y1 - 2013/7/1
N2 - Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Fucosterol, which is isolated from marine brown algae, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of fucosterol on photoaging and investigated its mechanisms of action in UV-irradiated immortalized human keratinocytes (HaCaT) by enzyme-linked immunosorbent assay, semi-quantitative reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that fucosterol attenuated UV-induced MMP and inflammatory cytokine expression by deactivating mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Fucosterol also increased type-I procollagen and antioxidant enzyme expression. Taken together, fucosterol regulates the expression of MMPs and type-I procollagen in UV-irradiated HaCaT by modulating MAPK, suggesting it as a potential candidate for prevention and treatment of skin aging.
AB - Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Fucosterol, which is isolated from marine brown algae, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of fucosterol on photoaging and investigated its mechanisms of action in UV-irradiated immortalized human keratinocytes (HaCaT) by enzyme-linked immunosorbent assay, semi-quantitative reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that fucosterol attenuated UV-induced MMP and inflammatory cytokine expression by deactivating mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Fucosterol also increased type-I procollagen and antioxidant enzyme expression. Taken together, fucosterol regulates the expression of MMPs and type-I procollagen in UV-irradiated HaCaT by modulating MAPK, suggesting it as a potential candidate for prevention and treatment of skin aging.
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UR - http://www.scopus.com/inward/citedby.url?scp=84879836936&partnerID=8YFLogxK
U2 - 10.1111/php.12061
DO - 10.1111/php.12061
M3 - Article
VL - 89
SP - 911
EP - 918
JO - Photochemistry and Photobiology
JF - Photochemistry and Photobiology
SN - 0031-8655
IS - 4
ER -