Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells

Myung Suk Kim, Ga Hui Oh, Min Ji Kim, Jae-Kwan Hwang

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Fucosterol, which is isolated from marine brown algae, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of fucosterol on photoaging and investigated its mechanisms of action in UV-irradiated immortalized human keratinocytes (HaCaT) by enzyme-linked immunosorbent assay, semi-quantitative reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that fucosterol attenuated UV-induced MMP and inflammatory cytokine expression by deactivating mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Fucosterol also increased type-I procollagen and antioxidant enzyme expression. Taken together, fucosterol regulates the expression of MMPs and type-I procollagen in UV-irradiated HaCaT by modulating MAPK, suggesting it as a potential candidate for prevention and treatment of skin aging.

Original languageEnglish
Pages (from-to)911-918
Number of pages8
JournalPhotochemistry and Photobiology
Volume89
Issue number4
DOIs
Publication statusPublished - 2013 Jul 1

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Collagen Type I
Matrix Metalloproteinases
antioxidants
matrices
cells
Skin
enzymes
Mitogen-Activated Protein Kinases
Assays
proteins
polymerase chain reaction
Antioxidants
Sunburn
Phaeophyta
algae
Skin Aging
collagens
Immunosorbents
radiation damage
ultraviolet radiation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

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title = "Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells",
abstract = "Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Fucosterol, which is isolated from marine brown algae, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of fucosterol on photoaging and investigated its mechanisms of action in UV-irradiated immortalized human keratinocytes (HaCaT) by enzyme-linked immunosorbent assay, semi-quantitative reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that fucosterol attenuated UV-induced MMP and inflammatory cytokine expression by deactivating mitogen-activated protein kinases (MAPKs) induced by reactive oxygen species. Fucosterol also increased type-I procollagen and antioxidant enzyme expression. Taken together, fucosterol regulates the expression of MMPs and type-I procollagen in UV-irradiated HaCaT by modulating MAPK, suggesting it as a potential candidate for prevention and treatment of skin aging.",
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Fucosterol inhibits matrix metalloproteinase expression and promotes type-1 procollagen production in UVB-induced HaCaT cells. / Kim, Myung Suk; Oh, Ga Hui; Kim, Min Ji; Hwang, Jae-Kwan.

In: Photochemistry and Photobiology, Vol. 89, No. 4, 01.07.2013, p. 911-918.

Research output: Contribution to journalArticle

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