Functional characterization of Caenorhabditis elegans DNA topoisomerase IIIα

You Chan Kim, Junho Lee, Hyeon Sook Koo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To investigate the function of a DNA topoisomerase III enzyme in Caenorhabditis elegans, the full-length cDNA of C. elegans DNA topoisomerase IIIα was cloned. The deduced amino acid sequence exhibited identities of 48 and 39% with those of human DNA topoisomerase IIIα and Saccharomyces cerevisiae DNA topoisomerase III, respectively. The overexpressed polypeptide showed an optimal activity for removing negative DNA supercoils at a relatively high temperature of 52-57°C, which is similar to the optimum temperatures of other eukaryotic DNA topoisomerase III enzymes. When topoisomerase IIIα expression was interfered with by a cognate double-stranded RNA injection, pleiotropic phenotypes with abnormalities in germ cell proliferation, oogenesis and embryogenesis appeared. These phenotypes were well correlated with mRNA expression localized in the meiotic cells of gonad and early embryonic cells.

Original languageEnglish
Pages (from-to)2012-2017
Number of pages6
JournalNucleic acids research
Volume28
Issue number9
DOIs
Publication statusPublished - 2000 May 1

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All Science Journal Classification (ASJC) codes

  • Genetics

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