Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide

Alavala Matta Reddy, Byung Kook Kwak, Hyung Jin Shim, Chiyoung Ahn, Sun Hang Cho, Byung Jin Kim, Sang Young Jeong, Sung Joo Hwang, Soon Hong Yuk

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 μ g Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.

Original languageEnglish
Pages (from-to)118-126
Number of pages9
JournalContrast Media and Molecular Imaging
Volume4
Issue number3
DOIs
Publication statusPublished - 2009 May 1

Fingerprint

Polyvinyls
Pyrrolidinones
Mesenchymal Stromal Cells
Nanoparticles
Bone Marrow
Cell Tracking
Chondrogenesis
Intramuscular Injections
Cell- and Tissue-Based Therapy
Contrast Media
Cell Movement
Cell Differentiation
Electron Microscopy
Stem Cells
Ischemia
Extremities
Magnetic Resonance Imaging
Staining and Labeling
Rabbits
Phenotype

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

Cite this

Reddy, Alavala Matta ; Kwak, Byung Kook ; Shim, Hyung Jin ; Ahn, Chiyoung ; Cho, Sun Hang ; Kim, Byung Jin ; Jeong, Sang Young ; Hwang, Sung Joo ; Yuk, Soon Hong. / Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide. In: Contrast Media and Molecular Imaging. 2009 ; Vol. 4, No. 3. pp. 118-126.
@article{db7d0a8f330c4b0183b577b2b2ce4b54,
title = "Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide",
abstract = "Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 μ g Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.",
author = "Reddy, {Alavala Matta} and Kwak, {Byung Kook} and Shim, {Hyung Jin} and Chiyoung Ahn and Cho, {Sun Hang} and Kim, {Byung Jin} and Jeong, {Sang Young} and Hwang, {Sung Joo} and Yuk, {Soon Hong}",
year = "2009",
month = "5",
day = "1",
doi = "10.1002/cmmi.271",
language = "English",
volume = "4",
pages = "118--126",
journal = "Contrast Media and Molecular Imaging",
issn = "1555-4309",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide. / Reddy, Alavala Matta; Kwak, Byung Kook; Shim, Hyung Jin; Ahn, Chiyoung; Cho, Sun Hang; Kim, Byung Jin; Jeong, Sang Young; Hwang, Sung Joo; Yuk, Soon Hong.

In: Contrast Media and Molecular Imaging, Vol. 4, No. 3, 01.05.2009, p. 118-126.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functional characterization of mesenchymal stem cells labeled with a novel PVP-coated superparamagnetic iron oxide

AU - Reddy, Alavala Matta

AU - Kwak, Byung Kook

AU - Shim, Hyung Jin

AU - Ahn, Chiyoung

AU - Cho, Sun Hang

AU - Kim, Byung Jin

AU - Jeong, Sang Young

AU - Hwang, Sung Joo

AU - Yuk, Soon Hong

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 μ g Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.

AB - Magnetic resonance imaging of cells labeled with superparamagnetic iron oxide (SPIO) could be a valuable tool for tracking transplanted cells in living organisms. Human bone marrow-derived mesenchymal stem cells (hBMMSC) were labeled with a novel polyvinyl pyrrolidone (PVP)-coated SPIO. Prussian blue staining and electron microscopy revealed that almost all of the cells were efficiently labeled with PVP-SPIO nanoparticles. There were no signs of cytotoxicity, even at concentrations of up to 1600 μ g Fe/ml of the nanoparticles, and the labeled cells were successfully visualized by in vitro cellular MRI. In addition, there was no significant alteration of the phenotype or the adipo/osteo/chondrogenic differentiation potential of the cells. This was in contrast to Feridex IV labeling that led to the inhibition of hBMMSC chondrogenesis. Following intramuscular injection in a rabbit hind limb ischemia model, the intercellular migration of the labeled cells toward the ablated site was clearly tracked through in vivo MRI. The localization of the transplanted cells observed by MRI correlated well with postmortem histological studies. These results demonstrate that the novel PVP-SPIO nanoparticles appear to be efficient MR contrast agents and may enable non-invasive in vivo tracking of stem cells in experimental and clinical settings during cell therapy.

UR - http://www.scopus.com/inward/record.url?scp=68949210572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949210572&partnerID=8YFLogxK

U2 - 10.1002/cmmi.271

DO - 10.1002/cmmi.271

M3 - Article

C2 - 19308999

AN - SCOPUS:68949210572

VL - 4

SP - 118

EP - 126

JO - Contrast Media and Molecular Imaging

JF - Contrast Media and Molecular Imaging

SN - 1555-4309

IS - 3

ER -