Functional characterization of NtCDPK1 in tobacco

Sang Sook Lee, Gyeong Mee Yoon, Eun Jung Rho, Eunpyo Moon, Hyun Sook Pai

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Abstract

We previously showed that NtCDPK1, a tobacco calcium-dependent protein kinase, interacts with and phosphorylates the Rpn3 regulatory subunit of the 26S proteasome, and that both NtCDPK1 and Rpn3 are mainly expressed in rapidly proliferating tissues, including shoot and root meristem. In this study, we examined NtCDPK1 expression in roots using GUS expression in transgenic Arabidopsis plants, and investigated its function in root development by generating transgenic tobacco plants carrying a sense NtCDPK1 transgene. GUS activity was first detected in roots two days after sowing. In later stages, strong GUS expression was detected in the root meristem and elongation zone, as well as the initiation sites and branch points of lateral roots. Transgenic tobacco plants in which NtCDPK1 expression was suppressed were smaller, and their root development was abnormal, with reduced lateral root formation and less elongation. These results suggest that NtCDPK1 plays a role in a signaling pathway regulating root development in tobacco.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalMolecules and cells
Volume21
Issue number1
Publication statusPublished - 2006 Feb 28

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Lee, S. S., Yoon, G. M., Rho, E. J., Moon, E., & Pai, H. S. (2006). Functional characterization of NtCDPK1 in tobacco. Molecules and cells, 21(1), 141-146.